Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605221522_f091 TAGGTAGACTATCCTAATCAAGAAACATGTAG(+CAGTGT)GATCCACGCTTATGTGGACTATAACCTGAGA NGG 10 8 17 8 24 0 100 5 10
    240605221520_xtfy TGCCTGTACCTTCAAATGTAAAGTAAGCCACG(+CAGTGT)GTACCTTGTGAGACATGTGTTCCTTGTGTTC NGG 10 8 17 8 24 0 100 5 10
    240605221518_ie49 CCAAGGAACACAAGGAACACATGTCTCACAAG(+CAGTGT)GTACCGTGGCTTACTTTACATTTGAAGGTAC NGG 10 8 17 8 24 0 100 5 10
    240605221517_59ir AGGTGACGGTCTGCTCATTTGTCAATGGGATG(+CAGTGT)GTACCTTCCTTTCTCTTCATGGATTGCATCC NGG 10 8 17 8 24 0 100 5 10
    240605221515_807j TGCAGGGATGCAATCCATGAAGAGAAAGGAAG(+CAGTGT)GTACCATCCCATTGACAAATGAGCAGACCGT NGG 10 8 17 8 24 0 100 5 10
    240605221514_4kcz ATGTTCTTTGTACCCACGCAGGGTTCTGTCTG(+CAGTGT)GTACCCCACATGTCAGCTTAAAGGACTTCTT NGG 10 8 17 8 24 0 100 5 10
    240605221512_xwkj AGTTAAAGAAGTCCTTTAAGCTGACATGTGGG(+CAGTGT)GTACCAGACAGAACCCTGCGTGGGTACAAAG NGG 10 8 17 8 24 0 100 5 10
    240605221510_ecu6 AGTGTCTGGGTTTGGTGGTTGTTTATGGGATG(+CAGTGT)GATCCCTAGGTAGTGCAGTCTCTGGATGGCC NGG 10 8 17 8 24 0 100 5 10
    240605221508_v0b8 GGAATGGCCATCCAGAGACTGCACTACCTAGG(+CAGTGT)GATCCATCCCATAAACAACCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605221506_wtou TACTGTTCCAGGTGTGGAGGGGGAATGGAGAG(+CAGTGT)GTACCTGGTAGGCATCCACAGGGGACATGAT NGG 10 8 17 8 24 0 100 5 10