Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605214223_8594 ATATCTATATTTGTGTACCATGTGCGCCCCTG(+CAGTGT)GTACCCAAGAAGATCAGAAGAGGGTGTCAGA NGG 10 8 17 8 24 0 100 5 10
    240605214222_7i92 CAAGATCTGACACCCTCTTCTGATCTTCTTGG(+CAGTGT)GTACCAGGGGCGCACATGGTACACAAATATA NGG 10 8 17 8 24 0 100 5 10
    240605214220_jl3v TGGGCAAGGAACAGGTGAAGGCTAGACTTTGG(+CAGTGT)GTACCAAACAGTCCTCGATCTGCAGATGCTG NGG 10 8 17 8 24 0 100 5 10
    240605214219_kg6p ACGCTCAGCATCTGCAGATCGAGGACTGTTTG(+CAGTGT)GTACCCAAAGTCTAGCCTTCACCTGTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240605214217_xmkg TTTCTTGATCTCAGAATAAATATTTCAAAGGG(+CAGTGT)GATCCAAAAATGCTGGACTTTTAAATATACT NGG 10 8 17 8 24 0 100 5 10
    240605214216_4f11 CTAAAAGTATATTTAAAAGTCCAGCATTTTTG(+CAGTGT)GATCCCCTTTGAAATATTTATTCTGAGATCA NGG 10 8 17 8 24 0 100 5 10
    240605214214_4jav ACAATGTTTGGAATGTTATATTGGGACAAATG(+CAGTGT)GTACCCAAGTGATCAGACATATAAAGTAGAC NGG 10 8 17 8 24 0 100 5 10
    240605214212_9li3 CATTTGTCTACTTTATATGTCTGATCACTTGG(+CAGTGT)GTACCATTTGTCCCAATATAACATTCCAAAC NGG 10 8 17 8 24 0 100 5 10
    240605214211_6kp3 AACCTTTCTCAGACCCACAAGTCCCAAACCTG(+CAGTGT)GATCCCAGTTTTATCCCATAATTTCTTCAAT NGG 10 8 17 8 24 0 100 5 10
    240605214209_lbuh GTTGTATTGAAGAAATTATGGGATAAAACTGG(+CAGTGT)GATCCAGGTTTGGGACTTGTGGGTCTGAGAA NGG 10 8 17 8 24 0 100 5 10