Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605220203_l0pq GTGTCCTGGATTTCCTGGATGTTTTGAGTTAG(+CAGTGT)GATCCTTTTGCATTTTGTATTTTCTTTGACT NGG 10 8 17 8 24 0 100 5 10
    240605220201_guoe ACAACAGTCAAAGAAAATACAAAATGCAAAAG(+CAGTGT)GATCCTAACTCAAAACATCCAGGAAATCCAG NGG 10 8 17 8 24 0 100 5 10
    240605220159_tnz3 AGGAGTTGTGTTCCACTCACCAGAGGTCTTAG(+CAGTGT)GATCCCGTGGGGAATCCTGTGTGGGCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605220157_6jxh ACCCGCACAGGCCCACACAGGATTCCCCACGG(+CAGTGT)GATCCTAAGACCTCTGGTGAGTGGAACACAA NGG 10 8 17 8 24 0 100 5 10
    240605220155_uty6 GGTGTCAGCGTTTGGATGCTGATTATGGAGTG(+CAGTGT)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605220153_8b1c GGATGGACCATGTAGAGACTGCCATATCCAGG(+CAGTGT)GATCCACTCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240605220152_9dlc TTCTGGATATATGCCCAAGAGAGGTATTGCTG(+CAGTGT)GATCCTCCGGTAGTACTATGTTCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605220149_bhuk TCCTCAGAAAATTGAACATAGTACTACCGGAG(+CAGTGT)GATCCAGCAATACCTCTCTTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240605220148_mtf0 AGCCTACTTGGGAGACTGCTGCTCTAAAGCGG(+CAGTGT)GTACCCAGACCAGTGAGGGTTTGCCAAAGCG NGG 10 8 17 8 24 0 100 5 10
    240605220146_0vbe GATGGCGCTTTGGCAAACCCTCACTGGTCTGG(+CAGTGT)GTACCCGCTTTAGAGCAGCAGTCTCCCAAGT NGG 10 8 17 8 24 0 100 5 10