Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605214400_7hu4 CTTGTAGACATTTATCTTCAGGCGTCATCTTG(+CAGTGT)GTACCAGTGGATCCCAGAGGACACACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240605214358_2e0a TTGGTCCTGGGTGTGTCCTCTGGGATCCACTG(+CAGTGT)GTACCAAGATGACGCCTGAAGATAAATGTCT NGG 10 8 17 8 24 0 100 5 10
    240605214356_rhu5 GTACATGGTGTTGAGGCACTGAGAGAACCAAG(+CAGTGT)GATCCACTCTCAGTTCCTACCCAGAAGGGTC NGG 10 8 17 8 24 0 100 5 10
    240605214355_t2kj TTCTGGACCCTTCTGGGTAGGAACTGAGAGTG(+CAGTGT)GATCCTTGGTTCTCTCAGTGCCTCAACACCA NGG 10 8 17 8 24 0 100 5 10
    240605214353_47ke GGCTTGCTGATTTCTAGTGTTCATCCCATTTG(+CAGTGT)GATCCTTAAAGGTACCCCATCCCAGGAAGAA NGG 10 8 17 8 24 0 100 5 10
    240605214352_j3bd GCACCTTCTTCCTGGGATGGGGTACCTTTAAG(+CAGTGT)GATCCAAATGGGATGAACACTAGAAATCAGC NGG 10 8 17 8 24 0 100 5 10
    240605214350_c85e GCCTTAGTCCTTCCCTCTTTAATCCCCTATGG(+CAGTGT)GATCCTTCTCTGTGTTCCTGTGTGTTTGCTT NGG 10 8 17 8 24 0 100 5 10
    240605214349_0cxf ATGAGAAGCAAACACACAGGAACACAGAGAAG(+CAGTGT)GATCCCATAGGGGATTAAAGAGGGAAGGACT NGG 10 8 17 8 24 0 100 5 10
    240605214348_to17 ACACTGAGGCTTCATTCTGTTCTTTAGCTTTG(+CAGTGT)GTACCTTTAATCCAGAGTCAATGGGAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605214347_ctp2 CTATATCTTTTCCCATTGACTCTGGATTAAAG(+CAGTGT)GTACCAAAGCTAAAGAACAGAATGAAGCCTC NGG 10 8 17 8 24 0 100 5 10