Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605214509_3si6 GCCCACTCCTGCAGACACAGCAGATCCAGCTG(+CAGTGT)GTACCCTAGCTGCCGCCCAGGGCAACCGGCC NGG 10 8 17 8 24 0 100 5 10
    240605214508_0dv7 AGACTGGCCGGTTGCCCTGGGCGGCAGCTAGG(+CAGTGT)GTACCAGCTGGATCTGCTGTGTCTGCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605214505_dlkz GGTTATCCGGGAAGTCCTGACAGGCCCCAGGG(+CAGTGT)GTACCAGCTGGGAGCAGGGTAACCTGGTGTC NGG 10 8 17 8 24 0 100 5 10
    240605214504_mesi GGAGGGACACCAGGTTACCCTGCTCCCAGCTG(+CAGTGT)GTACCCCTGGGGCCTGTCAGGACTTCCCGGA NGG 10 8 17 8 24 0 100 5 10
    240605214501_3iwd AGGGCTGAGTCTGAGGCACCATCTGGCCTCTG(+CAGTGT)GATCCCTGTGCCCGCCGGCCATGCCACCTCC NGG 10 8 17 8 24 0 100 5 10
    240605214500_nhe4 GGACAGGAGGTGGCATGGCCGGCGGGCACAGG(+CAGTGT)GATCCAGAGGCCAGATGGTGCCTCAGACTCA NGG 10 8 17 8 24 0 100 5 10
    240605214458_ms9s AGAATCCATTCTCTTTGCCCTTAAAGGTGAGG(+CAGTGT)GTACCCACTTCAGTTCATTGATGGGAACACC NGG 10 8 17 8 24 0 100 5 10
    240605214456_ixgo GACACGGTGTTCCCATCAATGAACTGAAGTGG(+CAGTGT)GTACCCTCACCTTTAAGGGCAAAGAGAATGG NGG 10 8 17 8 24 0 100 5 10
    240605214454_wh8o CTAAACTTCTAGCCCAACATGCATGTGCCCTG(+CAGTGT)GATCCAACTTCTAGCCCAACACAACTGAACA NGG 10 8 17 8 24 0 100 5 10
    240605214453_janx CGTTTTGTTCAGTTGTGTTGGGCTAGAAGTTG(+CAGTGT)GATCCAGGGCACATGCATGTTGGGCTAGAAG NGG 10 8 17 8 24 0 100 5 10