Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605214602_36f5 CCACCTGAGCCATATGCTGACCTCTTTGCCAG(+CAGTGT)GATCCTCCATGCTGCTGTCCAGTAGGGCCCA NGG 10 8 17 8 24 0 100 5 10
    240605214559_spso GGACATGGGCCCTACTGGACAGCAGCATGGAG(+CAGTGT)GATCCTGGCAAAGAGGTCAGCATATGGCTCA NGG 10 8 17 8 24 0 100 5 10
    240605214557_nq4t CTGCGCACCCCGCCGCCAAGCCCCAGCTCCAG(+CAGTGT)GTACCTACCGCGGTGCGCCTCTCCCGCCGGG NGG 10 8 17 8 24 0 100 5 10
    240605214556_6bzc CGGGGCCCGGCGGGAGAGGCGCACCGCGGTAG(+CAGTGT)GTACCTGGAGCTGGGGCTTGGCGGCGGGGTG NGG 10 8 17 8 24 0 100 5 10
    240605214553_75jq GATCCCTGATTCCGAGTCTGGGATTTCCCAGG(+CAGTGT)GATCCTGGATGAGCTCAAGACACCCCGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605214550_u0uf GTCTTTTCCCGGGGTGTCTTGAGCTCATCCAG(+CAGTGT)GATCCCTGGGAAATCCCAGACTCGGAATCAG NGG 10 8 17 8 24 0 100 5 10
    240605214549_w1bc ACTAACAGCTAACTGTAATTCCACTTCCAGAG(+CAGTGT)GATCCAACAGTATCTTCTGGCCTCTGACAAC NGG 10 8 17 8 24 0 100 5 10
    240605214547_mxhc GCAGTGTTGTCAGAGGCCAGAAGATACTGTTG(+CAGTGT)GATCCTCTGGAAGTGGAATTACAGTTAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605214545_9p9p CAGGGTGCCAGGGTCTGCTGAGGCTTTGGATG(+CAGTGT)GATCCAGGCTACTTGTCTACGATGTGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605214543_kvq4 TTGTCCCAGCCACATCGTAGACAAGTAGCCTG(+CAGTGT)GATCCATCCAAAGCCTCAGCAGACCCTGGCA NGG 10 8 17 8 24 0 100 5 10