Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605214653_hjt5 CTAGCAGGGGGCTTGTACAGTGAGTGATGGGG(+CAGTGT)GATCCCAGGAGAGTGCTGGTGATGGCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605214651_a57k CTCTGACCCAGCCATCACCAGCACTCTCCTGG(+CAGTGT)GATCCCCCATCACTCACTGTACAAGCCCCCT NGG 10 8 17 8 24 0 100 5 10
    240605214650_pe4a TACATCCTCATCACCAGTGCGGCTGGGTTCTG(+CAGTGT)GTACCAGCTTCAGCACCCCACAGTACCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605214648_70ve CTGAACCCTGGTACTGTGGGGTGCTGAAGCTG(+CAGTGT)GTACCAGAACCCAGCCGCACTGGTGATGAGG NGG 10 8 17 8 24 0 100 5 10
    240605214647_idfo GCCGCGCCATATCTCGCGGCCAGTTCCTGCAG(+CAGTGT)GTACCGGCTCGCCCTGCCAGTCGCAAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240605214645_ja61 CGGGGCTGGCTTGCGACTGGCAGGGCGAGCCG(+CAGTGT)GTACCTGCAGGAACTGGCCGCGAGATATGGC NGG 10 8 17 8 24 0 100 5 10
    240605214644_u1j9 AGACCCACCGAGGGGGAAGAGTGTGTCAAGGG(+CAGTGT)GTACCTAGGAGACCTGCTAAGCCGTCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605214642_7we0 CTGTTGCTGGGACGGCTTAGCAGGTCTCCTAG(+CAGTGT)GTACCCCTTGACACACTCTTCCCCCTCGGTG NGG 10 8 17 8 24 0 100 5 10
    240605214640_m4ph TTATAGTCCGTCATCCAGAGAAGTCAGGGCGG(+CAGTGT)GATCCTGGAGGCAAGGACTGAAGCAGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605214638_2z3q TCCACAGCCTCTGCTTCAGTCCTTGCCTCCAG(+CAGTGT)GATCCCGCCCTGACTTCTCTGGATGACGGAC NGG 10 8 17 8 24 0 100 5 10