Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605220620_fsi7 CTCTTATTAGAGTTACATTCACATTTGTAGTG(+CAGTGT)GATCCTGTCACTTTTTCATTCTAAGTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240605220619_ag1k TTCTAATTAAACTTAGAATGAAAAAGTGACAG(+CAGTGT)GATCCACTACAAATGTGAATGTAACTCTAAT NGG 10 8 17 8 24 0 100 5 10
    240605220617_l7vi ACATTATCATCACTGTTGTAGTTTGAAAGAGG(+CAGTGT)GTACCACTGTATAACCTATGATGGCTTCAAA NGG 10 8 17 8 24 0 100 5 10
    240605220616_4mf5 GCAAGTTTGAAGCCATCATAGGTTATACAGTG(+CAGTGT)GTACCCTCTTTCAAACTACAACAGTGATGAT NGG 10 8 17 8 24 0 100 5 10
    240605220614_w9el ATTGTCTTTAGACACACCAGGAGAGGACATCG(+CAGTGT)GATCCCCATTACAGATGGCTGTGAGCCACCA NGG 10 8 17 8 24 0 100 5 10
    240605220612_0ete CTACATGGTGGCTCACAGCCATCTGTAATGGG(+CAGTGT)GATCCGATGTCCTCTCCTGGTGTGTCTAAAG NGG 10 8 17 8 24 0 100 5 10
    240605220611_yfno CAATATTATTGGGAAACAGAGACTTTTTTCTG(+CAGTGT)GTACCAGACAAAGCTTAAATAGCCCTATATG NGG 10 8 17 8 24 0 100 5 10
    240605220610_5t7o TGCTTCATATAGGGCTATTTAAGCTTTGTCTG(+CAGTGT)GTACCAGAAAAAAGTCTCTGTTTCCCAATAA NGG 10 8 17 8 24 0 100 5 10
    240605220608_zknf TCCAGGGTTGCTGGCTCATGCCTGGACTCTGG(+CAGTGT)GATCCTGGCCCTGCAGTCCTCATTGGATATG NGG 10 8 17 8 24 0 100 5 10
    240605220605_kc1h TCTGGCATATCCAATGAGGACTGCAGGGCCAG(+CAGTGT)GATCCCAGAGTCCAGGCATGAGCCAGCAACC NGG 10 8 17 8 24 0 100 5 10