Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605214710_w6ei CCCAGGAGAAGAGGAACCTGGAGTCTAAAGGG(+CAGTGT)GATCCGACTTCCGGCAGAGTGGAGACTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605214709_ud6j CTTGTCTGCAGTCTCCACTCTGCCGGAAGTCG(+CAGTGT)GATCCCCTTTAGACTCCAGGTTCCTCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605214707_g5fb CCAGGAGAAGAGGAACCTGGAGTCTAAAAGGG(+CAGTGT)GATCCGACTTCCGGCAGAGTGGAGACTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605214705_i2u8 TGTCTGCAGAGTCTCCACTCTGCCGGAAGTCG(+CAGTGT)GATCCCCTTTTAGACTCCAGGTTCCTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605214704_0gxo CTTGTCTGCAGTCTCCACTCTGCCGGAAGTCG(+CAGTGT)GATCCCCTTTTAGACTCCAGTTTCCTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605214702_945a CCAGGAGAAGAGGAAACTGGAGTCTAAAAGGG(+CAGTGT)GATCCGACTTCCGGCAGAGTGGAGACTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605214701_49d9 CTTGTCTGCAGTCTCCACTCTGCCGGAAGTCG(+CAGTGT)GATCCCCTTTTAGACTCCAGGTTCCTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605214659_suwg CCAGGAGAAGAGGAACCTGGAGTCTAAAAGGG(+CAGTGT)GATCCGACTTCCGGCAGAGTGGAGACTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605214657_zg84 TCCTCCCTCTGGGTTCATCCTGACCAATCAGG(+CAGTGT)GTACCCTGAGTTCCTACCCAGTCCCCTTTCA NGG 10 8 17 8 24 0 100 5 10
    240605214656_445s CAGTATGAAAGGGGACTGGGTAGGAACTCAGG(+CAGTGT)GTACCCTGATTGGTCAGGATGAACCCAGAGG NGG 10 8 17 8 24 0 100 5 10