Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605214822_6vrk GAGGGGGGTGTGAAAGCCCACAAACCTTCCTG(+CAGTGT)GATCCCAGCCTCCCTTCACTGGGGTACAGAC NGG 10 8 17 8 24 0 100 5 10
    240605214820_tqkx TGGGTGTCTGTACCCCAGTGAAGGGAGGCTGG(+CAGTGT)GATCCAGGAAGGTTTGTGGGCTTTCACACCC NGG 10 8 17 8 24 0 100 5 10
    240605214819_w05u GCATGTTAATGCTCCCCCCCACCCCGGCTTTG(+CAGTGT)GTACCGGGGGGGGGGGCAGGAAACTGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605214816_ex76 CCCCGTGCCTCAGTTTCCTGCCCCCCCCCCCG(+CAGTGT)GTACCAAAGCCGGGGTGGGGGGGAGCATTAA NGG 10 8 17 8 24 0 100 5 10
    240605214815_m45n ACACCGTTCAATTTTGCACATATGTCCTCATG(+CAGTGT)GATCCCCAGTTTTGTCCCTCCACTTGGGCAC NGG 10 8 17 8 24 0 100 5 10
    240605214813_s3ey CTCGTGTGCCCAAGTGGAGGGACAAAACTGGG(+CAGTGT)GATCCATGAGGACATATGTGCAAAATTGAAC NGG 10 8 17 8 24 0 100 5 10
    240605214811_8c20 CCTTTGAGACAGAGCAATGATCTAAGACATAG(+CAGTGT)GTACCTCCATTGGGCAGGTCCCCCAGTTGAT NGG 10 8 17 8 24 0 100 5 10
    240605214810_z1wg GCCAGATCAACTGGGGGACCTGCCCAATGGAG(+CAGTGT)GTACCTATGTCTTAGATCATTGCTCTGTCTC NGG 10 8 17 8 24 0 100 5 10
    240605214808_5q07 AGGCCATGTTCCTGTGGGAGGGGCTGTGGAAG(+CAGTGT)GATCCACACCGGGCTGTTTCCATCTGGAAAC NGG 10 8 17 8 24 0 100 5 10
    240605214807_jodk GCTTCGTTTCCAGATGGAAACAGCCCGGTGTG(+CAGTGT)GATCCTTCCACAGCCCCTCCCACAGGAACAT NGG 10 8 17 8 24 0 100 5 10