Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605214857_bqgs TATCATAGAAGCTAGGTCTGAATCAAATCTTG(+CAGTGT)GTACCTCAGAGGAAGTAGGAAGCGCCCACTG NGG 10 8 17 8 24 0 100 5 10
    240605214855_bl4v CAATCCAGTGGGCGCTTCCTACTTCCTCTGAG(+CAGTGT)GTACCAAGATTTGATTCAGACCTAGCTTCTA NGG 10 8 17 8 24 0 100 5 10
    240605214854_syp2 TTGCCTCCCAAGGGCTAATGTTAAAGGTGTGG(+CAGTGT)GTACCATCACTCCCAGGCAAATCTTCCATTT NGG 10 8 17 8 24 0 100 5 10
    240605214852_4usm TCCAAAAATGGAAGATTTGCCTGGGAGTGATG(+CAGTGT)GTACCCACACCTTTAACATTAGCCCTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605214851_argw CTATGGGCCCTTTGGTGACATGTGGGGCTCTG(+CAGTGT)GATCCTTGGGTGTCCTCACTGTGGCCACTCT NGG 10 8 17 8 24 0 100 5 10
    240605214848_qbhf CAGGCAGAGTGGCCACAGTGAGGACACCCAAG(+CAGTGT)GATCCAGAGCCCCACATGTCACCAAAGGGCC NGG 10 8 17 8 24 0 100 5 10
    240605214845_oagx TGTTGCTTTCGGGACCCAAAGACTCTTCCGGG(+CAGTGT)GATCCAGCATCTTGCCTTCCATTCTCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605214844_w3hl CAGGCAGAAAGAGAATGGAAGGCAAGATGCTG(+CAGTGT)GATCCCCGGAAGAGTCTTTGGGTCCCGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605214842_3omv CACAGATGAGAATCTAACTTGGACCCCACCTG(+CAGTGT)GTACCCCACCTGGTACACTGCACATCAGTGT NGG 10 8 17 8 24 0 100 5 10
    240605214840_dah7 AAAGGACACTGATGTGCAGTGTACCAGGTGGG(+CAGTGT)GTACCAGGTGGGGTCCAAGTTAGATTCTCAT NGG 10 8 17 8 24 0 100 5 10