Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605214950_g924 CTAGCTCCAGACCCTTCTCTACAGATGTTGTG(+CAGTGT)GTACCCTCCAGCCTCCAAGCCACACAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240605214948_2as9 TGTGCCTGACTGTGTGGCTTGGAGGCTGGAGG(+CAGTGT)GTACCACAACATCTGTAGAGAAGGGTCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605214945_u8o3 TTCCCCACTTCCTCACAAAGCCCTGACCCCAG(+CAGTGT)GATCCCAGCGATGGACAGGAACACCTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240605214943_7fox GTAGACTCTGAGGTGTTCCTGTCCATCGCTGG(+CAGTGT)GATCCTGGGGTCAGGGCTTTGTGAGGAAGTG NGG 10 8 17 8 24 0 100 5 10
    240605214941_xjb2 TGGCATGGTCATGTGCCCTCTGGCCTGTGGAG(+CAGTGT)GTACCTATAGTGGCAAGGACAGGGGTCAGGT NGG 10 8 17 8 24 0 100 5 10
    240605214940_0scd CTCCCACCTGACCCCTGTCCTTGCCACTATAG(+CAGTGT)GTACCTCCACAGGCCAGAGGGCACATGACCA NGG 10 8 17 8 24 0 100 5 10
    240605214938_nk73 CCTGGAACAGGATATCAATGAAGCAGCTGAGG(+CAGTGT)GTACCAAGAGTGTGGGGTAAGGATTTCATTC NGG 10 8 17 8 24 0 100 5 10
    240605214936_07be CCCTTGAATGAAATCCTTACCCCACACTCTTG(+CAGTGT)GTACCCTCAGCTGCTTCATTGATATCCTGTT NGG 10 8 17 8 24 0 100 5 10
    240605214934_hkkg CTGAGGAAGCTACAAAGTGTGTTCTCTCCAGG(+CAGTGT)GTACCTTCCAATGCTTCCCTAGCTTCTTGGC NGG 10 8 17 8 24 0 100 5 10
    240605214932_b7o8 TGTGGGCCAAGAAGCTAGGGAAGCATTGGAAG(+CAGTGT)GTACCCTGGAGAGAACACACTTTGTAGCTTC NGG 10 8 17 8 24 0 100 5 10