Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215056_fpqm AGGGCTGATGGCCCTGGGGTTCCTTGTCAAAG(+CAGTGT)GATCCGCCTGTGTCTTTATTAACACAGTTCT NGG 10 8 17 8 24 0 100 5 10
    240605215054_1c4j AACCCAGAACTGTGTTAATAAAGACACAGGCG(+CAGTGT)GATCCTTTGACAAGGAACCCCAGGGCCATCA NGG 10 8 17 8 24 0 100 5 10
    240605215053_ko46 GAGCCACCCTGAATCGTCTCCAGAGTGTCATG(+CAGTGT)GATCCAGGCTGGCTTCTCACTTGCTGTATAG NGG 10 8 17 8 24 0 100 5 10
    240605215051_tk4e CTATGCTATACAGCAAGTGAGAAGCCAGCCTG(+CAGTGT)GATCCATGACACTCTGGAGACGATTCAGGGT NGG 10 8 17 8 24 0 100 5 10
    240605215050_skyx TTTGTTTGCATGTATGTATGTGTGCATGCTTG(+CAGTGT)GTACCTAGAGTTAAGGGCAGCAGACACCCCT NGG 10 8 17 8 24 0 100 5 10
    240605215049_8zdj ACTCCAGGGGTGTCTGCTGCCCTTAACTCTAG(+CAGTGT)GTACCAAGCATGCACACATACATACATGCAA NGG 10 8 17 8 24 0 100 5 10
    240605215048_fs4p CCTGCCCAGCCCCTCCTCCCACAGGAACACAG(+CAGTGT)GTACCACCTGCACCCACGACAGCTTCTTTTC NGG 10 8 17 8 24 0 100 5 10
    240605215046_8bo1 TGTGAGAAAAGAAGCTGTCGTGGGTGCAGGTG(+CAGTGT)GTACCTGTGTTCCTGTGGGAGGAGGGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605215045_vfxd GTGAGTGTGTGGCTTGCTGTCGTGTCCTCTGG(+CAGTGT)GATCCCAAGCCACGCGGGTCTTCCCTCTGTA NGG 10 8 17 8 24 0 100 5 10
    240605215043_t3i6 CCATCTACAGAGGGAAGACCCGCGTGGCTTGG(+CAGTGT)GATCCCAGAGGACACGACAGCAAGCCACACA NGG 10 8 17 8 24 0 100 5 10