Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215021_mchw TGTCTCGGTCCCGGCCGCTGCTCTCCAGCCAG(+CAGTGT)GTACCCTCCTCACTCCCTGCTCGCGCAGTTC NGG 10 8 17 8 24 0 100 5 10
    240605215019_3397 AGTGCGAACTGCGCGAGCAGGGAGTGAGGAGG(+CAGTGT)GTACCTGGCTGGAGAGCAGCGGCCGGGACCG NGG 10 8 17 8 24 0 100 5 10
    240605215018_0mvb GGCGCCGGGGGCGGTGCCGGCTCCAATCACGG(+CAGTGT)GATCCGCCCAAGGGCCCCAACGCGGTAGGCG NGG 10 8 17 8 24 0 100 5 10
    240605215016_8lib GGAAGCGCCTACCGCGTTGGGGCCCTTGGGCG(+CAGTGT)GATCCCGTGATTGGAGCCGGCACCGCCCCCG NGG 10 8 17 8 24 0 100 5 10
    240605215014_7p2d TCCTTCTTGCTCCGCTTCTATTCTGTCAAGGG(+CAGTGT)GATCCCTGCAGTCTGAGGCTTCCCCCCCAAA NGG 10 8 17 8 24 0 100 5 10
    240605215012_371i CCAGGTTTGGGGGGGAAGCCTCAGACTGCAGG(+CAGTGT)GATCCCCTTGACAGAATAGAAGCGGAGCAAG NGG 10 8 17 8 24 0 100 5 10
    240605215010_giz4 TGGGTCCCAATGCATCTGCTGGGGGCTGAGAG(+CAGTGT)GTACCTTCTGCCCATCACCAGCCTTGTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605215009_835p CAGCACAGAACAAGGCTGGTGATGGGCAGAAG(+CAGTGT)GTACCTCTCAGCCCCCAGCAGATGCATTGGG NGG 10 8 17 8 24 0 100 5 10
    240605215008_d1o1 TTCCTTTACTGAAGGAGGGCCAAGTCAGCATG(+CAGTGT)GTACCACTGAGACAGATTTTTTTTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605215007_s9xr TGTTTAAAAAAAAAAAAAAATCTGTCTCAGTG(+CAGTGT)GTACCATGCTGACTTGGCCCTCCTTCAGTAA NGG 10 8 17 8 24 0 100 5 10