Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215127_8o89 ATGCAGGTTAAGTGCCTAAGGTTTCCTGTTGG(+CAGTGT)GTACCACATGCTTCTCTAAAAGGCTTTAAGG NGG 10 8 17 8 24 0 100 5 10
    240605215125_izxw GACTCCCTTAAAGCCTTTTAGAGAAGCATGTG(+CAGTGT)GTACCCAACAGGAAACCTTAGGCACTTAACC NGG 10 8 17 8 24 0 100 5 10
    240605215124_rbeh CACTAACTACTGTGACAAGAAACTTAGCAAAG(+CAGTGT)GTACCTGTGTGTACAGTGAGGGCAGCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605215122_omn8 CTCATAGCCAGCTGCCCTCACTGTACACACAG(+CAGTGT)GTACCTTTGCTAAGTTTCTTGTCACAGTAGT NGG 10 8 17 8 24 0 100 5 10
    240605215121_4owc AGTCGTCCCTCTGTCAGCTTGGAGCGTCTCAG(+CAGTGT)GTACCCAGGCAGTCTCAGACTTCTGGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240605215119_wb34 TTTCGGAGGCCCAGAAGTCTGAGACTGCCTGG(+CAGTGT)GTACCTGAGACGCTCCAAGCTGACAGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605215117_6yjq GAGGTGAGCGGGGCTGATCTATACCAAGCCAG(+CAGTGT)GATCCTTCTGAATTAGATTCTTGTCTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605215116_ii9j AACTGTCCCAAGACAAGAATCTAATTCAGAAG(+CAGTGT)GATCCTGGCTTGGTATAGATCAGCCCCGCTC NGG 10 8 17 8 24 0 100 5 10
    240605215114_lqcj AAGTGACAAGCTGACTGTGGCATTGCACACTG(+CAGTGT)GATCCAAAGAGAAGGTTGTAAAAGAGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605215113_1cxv ACCAACCCAGCTCTTTTACAACCTTCTCTTTG(+CAGTGT)GATCCAGTGTGCAATGCCACAGTCAGCTTGT NGG 10 8 17 8 24 0 100 5 10