Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215042_dky0 GAAGCCAGTTTCCTAAACACCATGCAGAAGTG(+CAGTGT)GTACCCTGGGGCCTGGAAGCTCAAGCTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605215039_9if1 CACCTAGGAAGCTTGAGCTTCCAGGCCCCAGG(+CAGTGT)GTACCACTTCTGCATGGTGTTTAGGAAACTG NGG 10 8 17 8 24 0 100 5 10
    240605215037_pkgz TTCTGGAGCCTGGTTACTCCTTAGAATTCAAG(+CAGTGT)GATCCAAGTGCAAGAGCTTCAGGCTTCAGCT NGG 10 8 17 8 24 0 100 5 10
    240605215036_qrx7 CTGACAGCTGAAGCCTGAAGCTCTTGCACTTG(+CAGTGT)GATCCTTGAATTCTAAGGAGTAACCAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605215034_s9k7 GGGAGGGTCTGCCCCCATTTTGACAGGCCAAG(+CAGTGT)GATCCTGGGACCACTTGGGGTTGCAGTGTAT NGG 10 8 17 8 24 0 100 5 10
    240605215031_43g9 AGGTCATACACTGCAACCCCAAGTGGTCCCAG(+CAGTGT)GATCCTTGGCCTGTCAAAATGGGGGCAGACC NGG 10 8 17 8 24 0 100 5 10
    240605215030_zc3j GGTGTCATTGCTTGGAGGCTAATTATGGGATG(+CAGTGT)GATCCCTGGATATGGCAGTCTCTAGATGGAC NGG 10 8 17 8 24 0 100 5 10
    240605215027_0r97 GGATGGTCCATCTAGAGACTGCCATATCCAGG(+CAGTGT)GATCCATCCCATAATTAGCCTCCAAGCAATG NGG 10 8 17 8 24 0 100 5 10
    240605215025_09od GGTGCACAGGCCTCGGAAGGCAGAGGCAGGGG(+CAGTGT)GATCCCTACCGGTTTAGAATCTGCATGGGCT NGG 10 8 17 8 24 0 100 5 10
    240605215023_spqz TATGCAGCCCATGCAGATTCTAAACCGGTAGG(+CAGTGT)GATCCCCCTGCCTCTGCCTTCCGAGGCCTGT NGG 10 8 17 8 24 0 100 5 10