Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215144_1w97 TCGAGATCCAGTGGAAGCTAGAAAGTGCACAG(+CAGTGT)GATCCCAGAGCTACAGCCTGGCAGAAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240605215143_ko1a AGAGGATGACTTCTGCCAGGCTGTAGCTCTGG(+CAGTGT)GATCCTGTGCACTTTCTAGCTTCCACTGGAT NGG 10 8 17 8 24 0 100 5 10
    240605215141_2274 CCGCTTTGTGAGAGCATCAGGCCCTGTGTAGG(+CAGTGT)GATCCCCAGCAGTAACACTTGTCAGTTTACT NGG 10 8 17 8 24 0 100 5 10
    240605215138_oyxp GGACCAGTAAACTGACAAGTGTTACTGCTGGG(+CAGTGT)GATCCCTACACAGGGCCTGATGCTCTCACAA NGG 10 8 17 8 24 0 100 5 10
    240605215137_gm2p TCGAGATCCAGTGGAAGCTAGAAAGTGCACAG(+CAGTGT)GATCCCAGAGCTACAGCCTGGCAGAAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240605215134_35zc AGAGGATGACTTCTGCCAGGCTGTAGCTCTGG(+CAGTGT)GATCCTGTGCACTTTCTAGCTTCCACTGGAT NGG 10 8 17 8 24 0 100 5 10
    240605215133_y7pv GGGCTGTCTCTGACCCTATTACCTGCCTTTGG(+CAGTGT)GATCCATTCCCCCTCCTGCATCGTCCAACAT NGG 10 8 17 8 24 0 100 5 10
    240605215131_om0y TACTAATGTTGGACGATGCAGGAGGGGGAATG(+CAGTGT)GATCCCAAAGGCAGGTAATAGGGTCAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240605215130_k5kr GCCCTCTTTCCTTGGCACATTCCATGTAGTTG(+CAGTGT)GTACCCTTGTGGGGACCCACAGCATCCATGG NGG 10 8 17 8 24 0 100 5 10
    240605215128_s605 GGTTTCCATGGATGCTGTGGGTCCCCACAAGG(+CAGTGT)GTACCAACTACATGGAATGTGCCAAGGAAAG NGG 10 8 17 8 24 0 100 5 10