Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605221017_6rx7 AAACCCTGTATCCATAATAACTTCAGTTCTTG(+CAGTGT)GATCCAGGGCTTCTGACAAAGTGAATGATGA NGG 10 8 17 8 24 0 100 5 10
    240605221015_e5mv TTGCTTCATCATTCACTTTGTCAGAAGCCCTG(+CAGTGT)GATCCAAGAACTGAAGTTATTATGGATACAG NGG 10 8 17 8 24 0 100 5 10
    240605221013_nqtx TATTTTTAAAATTGTATTGCTGCTCAAGAATG(+CAGTGT)GTACCCTTTTGTCAAAAAGGTAGATATTCAT NGG 10 8 17 8 24 0 100 5 10
    240605221011_vqha CAATTATGAATATCTACCTTTTTGACAAAAGG(+CAGTGT)GTACCATTCTTGAGCAGCAATACAATTTTAA NGG 10 8 17 8 24 0 100 5 10
    240605221010_8av5 TCGAACAGGGTGGGGGAAGGAGGAGGTCAGAG(+CAGTGT)GATCCGAGAAGGTGATCATTCAACTTCCAAA NGG 10 8 17 8 24 0 100 5 10
    240605221008_n4iq CCAGGTTTGGAAGTTGAATGATCACCTTCTCG(+CAGTGT)GATCCTCTGACCTCCTCCTTCCCCCACCCTG NGG 10 8 17 8 24 0 100 5 10
    240605221006_0mz8 GGCCTAACAAGGTCACCCTCAGATCTTCCAAG(+CAGTGT)GTACCCATGGGAACCATGGTCTAGCACAGTG NGG 10 8 17 8 24 0 100 5 10
    240605221004_n1cc AGAACCACTGTGCTAGACCATGGTTCCCATGG(+CAGTGT)GTACCTTGGAAGATCTGAGGGTGACCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605221002_mh0i CATAGCCCAGGATGGACAGGAGTACGTGGGAG(+CAGTGT)GTACCTCCAAACACAGAGGTCCATGCGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605221001_as1s GCACGCCCACGCATGGACCTCTGTGTTTGGAG(+CAGTGT)GTACCTCCCACGTACTCCTGTCCATCCTGGG NGG 10 8 17 8 24 0 100 5 10