Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215257_isyl CATGTCTCTTAAGTTATTGTGACCTACACTGG(+CAGTGT)GTACCGGAGGGGATGGGGGATGGCTTCAGCT NGG 10 8 17 8 24 0 100 5 10
    240605215254_y4ez ACAGCAGCTGAAGCCATCCCCCATCCCCTCCG(+CAGTGT)GTACCCAGTGTAGGTCACAATAACTTAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605215252_e3ho TGTGGCACTCAAGCATACTGATCCCCGCTCAG(+CAGTGT)GTACCACCATTCCCTATGTAGAGGAGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605215250_w3tn GGGTGCTTCCCTCCTCTACATAGGGAATGGTG(+CAGTGT)GTACCTGAGCGGGGATCAGTATGCTTGAGTG NGG 10 8 17 8 24 0 100 5 10
    240605215249_sji8 ATAGAACACTCAAAAGGGACGTGAATGTGCAG(+CAGTGT)GTACCATGGGTCTGGGGTGACCTGCTTCACA NGG 10 8 17 8 24 0 100 5 10
    240605215247_u5yt ACTTCTGTGAAGCAGGTCACCCCAGACCCATG(+CAGTGT)GTACCTGCACATTCACGTCCCTTTTGAGTGT NGG 10 8 17 8 24 0 100 5 10
    240605215245_8ggl GCTGCCTGAGGGAGCCTTTGGAGAGCTAACTG(+CAGTGT)GTACCCTGATTATAGTCCATTAAGAATCACT NGG 10 8 17 8 24 0 100 5 10
    240605215244_lmoq ACCTAAGTGATTCTTAATGGACTATAATCAGG(+CAGTGT)GTACCAGTTAGCTCTCCAAAGGCTCCCTCAG NGG 10 8 17 8 24 0 100 5 10
    240605215242_ghyj AGACACAGGTGCAGAGCGGGAAGGCTGACTGG(+CAGTGT)GATCCCGCCACCAGCTGTTTTCCCTCTTGTC NGG 10 8 17 8 24 0 100 5 10
    240605215240_gqwn GAAATGACAAGAGGGAAAACAGCTGGTGGCGG(+CAGTGT)GATCCCAGTCAGCCTTCCCGCTCTGCACCTG NGG 10 8 17 8 24 0 100 5 10