Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215332_oa14 AGGCTCCACACACCAGGTATTGCTGATTCCAG(+CAGTGT)GTACCACCCTTGACATGTGTGTCTGAGTCCC NGG 10 8 17 8 24 0 100 5 10
    240605215330_jop3 AGGCTGGGACTCAGACACACATGTCAAGGGTG(+CAGTGT)GTACCTGGAATCAGCAATACCTGGTGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605215328_b2n9 CAGCTTTGTCAACAGTTCTGTATCCATACCTG(+CAGTGT)GTACCCTCGGCCCCGCTTCCGAGTCGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240605215326_aquu AAGTGAAAAGCGACTCGGAAGCGGGGCCGAGG(+CAGTGT)GTACCAGGTATGGATACAGAACTGTTGACAA NGG 10 8 17 8 24 0 100 5 10
    240605215324_4le6 AGAAGACACCCAGGGTGACGTCTCATCCTCAG(+CAGTGT)GATCCCCCACGTCTGCTGCCATCACAGCCGC NGG 10 8 17 8 24 0 100 5 10
    240605215322_pbmg GGGATGCGGCTGTGATGGCAGCAGACGTGGGG(+CAGTGT)GATCCTGAGGATGAGACGTCACCCTGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240605215321_u68x ACAGGGGTAAAGGTTAGACAGTGGCAACTTAG(+CAGTGT)GATCCTGTTAGGGTCTGGCCCATGTTTTAAC NGG 10 8 17 8 24 0 100 5 10
    240605215319_hgpl TGGGAGTTAAAACATGGGCCAGACCCTAACAG(+CAGTGT)GATCCTAAGTTGCCACTGTCTAACCTTTACC NGG 10 8 17 8 24 0 100 5 10
    240605215318_5gmh TAACTCATGCCCAGACACTGATGGAGGGGCAG(+CAGTGT)GTACCCCCAAAACACCAAGCCCTAAGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240605215316_qajd GGTAGGGCCTCTTAGGGCTTGGTGTTTTGGGG(+CAGTGT)GTACCTGCCCCTCCATCAGTGTCTGGGCATG NGG 10 8 17 8 24 0 100 5 10