Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605220738_0t3n GCCGGTTTCCGGGCTGGCCCCTCCTACCCCTG(+CAGTGT)GATCCCAGACTGTCCTGGATTCCACCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605220736_girg ATGAACCCAGGGTGGAATCCAGGACAGTCTGG(+CAGTGT)GATCCAGGGGTAGGAGGGGCCAGCCCGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605220734_fuod GGTCCCTGCAGGAGGACCCTCACCTTCACCTG(+CAGTGT)GATCCGCCTTGGCCTCTACCCTTGCCCAACC NGG 10 8 17 8 24 0 100 5 10
    240605220733_wiss TGGGAGGTTGGGCAAGGGTAGAGGCCAAGGCG(+CAGTGT)GATCCAGGTGAAGGTGAGGGTCCTCCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605220731_hc7y TAACAGGCCACATTCTGATCTTCCAACCGCTG(+CAGTGT)GATCCCTTCCTCCACCTGGCTTCTCAGCTCC NGG 10 8 17 8 24 0 100 5 10
    240605220729_shrg CATGGGGAGCTGAGAAGCCAGGTGGAGGAAGG(+CAGTGT)GATCCAGCGGTTGGAAGATCAGAATGTGGCC NGG 10 8 17 8 24 0 100 5 10
    240605220728_tvgo AAGAGGAGATTTTTTACAAAGTTTCAGTGTGG(+CAGTGT)GATCCCTAGGCACTGCCATCACTCAGCAAGA NGG 10 8 17 8 24 0 100 5 10
    240605220725_jwkt TGACTTCTTGCTGAGTGATGGCAGTGCCTAGG(+CAGTGT)GATCCCACACTGAAACTTTGTAAAAAATCTC NGG 10 8 17 8 24 0 100 5 10
    240605220724_abpc TGGCGCCAACATGACCAGAGTCACTAGGAAAG(+CAGTGT)GATCCTAGGTAGCACAGACACAAACCATGTC NGG 10 8 17 8 24 0 100 5 10
    240605220722_4xfk CCCAGGACATGGTTTGTGTCTGTGCTACCTAG(+CAGTGT)GATCCTTTCCTAGTGACTCTGGTCATGTTGG NGG 10 8 17 8 24 0 100 5 10