Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215423_vdmy GCACACAGGTATCTCAACAGAGCTGGCACAGG(+CAGTGT)GTACCTTTTTTTAAAGGGGCAGCTAGAATCC NGG 10 8 17 8 24 0 100 5 10
    240605215422_vc2e ACTAAGGATTCTAGCTGCCCCTTTAAAAAAAG(+CAGTGT)GTACCCTGTGCCAGCTCTGTTGAGATACCTG NGG 10 8 17 8 24 0 100 5 10
    240605215420_v5iy ACATTACTGTTGTACCCAGTGACTTCACTTGG(+CAGTGT)GATCCCTGTCTCCTTAAACCACATATAATTA NGG 10 8 17 8 24 0 100 5 10
    240605215418_qown ACTAATAATTATATGTGGTTTAAGGAGACAGG(+CAGTGT)GATCCCAAGTGAAGTCACTGGGTACAACAGT NGG 10 8 17 8 24 0 100 5 10
    240605215416_8zex TTAAAAAGAGAATTACCCATACGGAAGAGGAG(+CAGTGT)GATCCTAACAATGCTTGTTCTGGCTGTTGCT NGG 10 8 17 8 24 0 100 5 10
    240605215415_uget AGTCAAGCAACAGCCAGAACAAGCATTGTTAG(+CAGTGT)GATCCTCCTCTTCCGTATGGGTAATTCTCTT NGG 10 8 17 8 24 0 100 5 10
    240605215414_dpdo GTTTCAGCAGTCCTGGAAAATGTGCTTTCTTG(+CAGTGT)GATCCAAATTACATTCTCTTCCTAAACCAAA NGG 10 8 17 8 24 0 100 5 10
    240605215413_nn7c ACAGTTTTGGTTTAGGAAGAGAATGTAATTTG(+CAGTGT)GATCCAAGAAAGCACATTTTCCAGGACTGCT NGG 10 8 17 8 24 0 100 5 10
    240605215411_j58y GGGTCTCTAGATAACGCTACCCTGAATCAGAG(+CAGTGT)GATCCCTAAACCCAATGGCATTAACTGCACA NGG 10 8 17 8 24 0 100 5 10
    240605215410_s2ru CTTCTTGTGCAGTTAATGCCATTGGGTTTAGG(+CAGTGT)GATCCTCTGATTCAGGGTAGCGTTATCTAGA NGG 10 8 17 8 24 0 100 5 10