Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215438_zg89 TATTTTGACATCACCAACTCAAAATGCTAACG(+CAGTGT)GTACCACTAATTGTATGCAATCTGGAATGAT NGG 10 8 17 8 24 0 100 5 10
    240605215437_tkzv TTAAGATCATTCCAGATTGCATACAATTAGTG(+CAGTGT)GTACCGTTAGCATTTTGAGTTGGTGATGTCA NGG 10 8 17 8 24 0 100 5 10
    240605215435_adg0 AGACTTCCCTTCTACCTCAAGTGCATTTCCAG(+CAGTGT)GATCCTTTTGAAGAGAAAATTCAGAGCCTTA NGG 10 8 17 8 24 0 100 5 10
    240605215433_q0vq TAAAGTAAGGCTCTGAATTTTCTCTTCAAAAG(+CAGTGT)GATCCTGGAAATGCACTTGAGGTAGAAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605215432_1io7 GGTGATTCTGCTTTAAATGAGTTCCTCAGAAG(+CAGTGT)GATCCGATATCAGCTAGTTTAGGAAGCATGG NGG 10 8 17 8 24 0 100 5 10
    240605215431_t27t AATTTCCATGCTTCCTAAACTAGCTGATATCG(+CAGTGT)GATCCTTCTGAGGAACTCATTTAAAGCAGAA NGG 10 8 17 8 24 0 100 5 10
    240605215428_u9rt GTCCACAGCTCTCTGTAACTCCAGTGCCAAGG(+CAGTGT)GATCCAACACACTCTTCTAACCTCTGTGGAC NGG 10 8 17 8 24 0 100 5 10
    240605215427_0ns6 CTGGTGTCCACAGAGGTTAGAAGAGTGTGTTG(+CAGTGT)GATCCCTTGGCACTGGAGTTACAGAGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605215426_5wx5 CACAAGTTAAGGAAAGATATTAGCCAAAGAAG(+CAGTGT)GTACCAGCCTATGAAAAACAAATTAAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605215424_yvwn CTGGTACTTTTTAATTTGTTTTTCATAGGCTG(+CAGTGT)GTACCTTCTTTGGCTAATATCTTTCCTTAAC NGG 10 8 17 8 24 0 100 5 10