Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215545_ndnx TTCTACACAGAATATTTTCCCCATATTTATTG(+CAGTGT)GATCCAACCTGCTTACGGGATCTGTTAGTGC NGG 10 8 17 8 24 0 100 5 10
    240605215543_1ql1 TTACAGCACTAACAGATCCCGTAAGCAGGTTG(+CAGTGT)GATCCAATAAATATGGGGAAAATATTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605215542_8ndr CTGTGGAGCATTTTCTTGATTGCTAATTGGAG(+CAGTGT)GATCCAACCCACCGTGGGTGTTACTATCCCT NGG 10 8 17 8 24 0 100 5 10
    240605215540_oqqf TGCTTAGGGATAGTAACACCCACGGTGGGTTG(+CAGTGT)GATCCTCCAATTAGCAATCAAGAAAATGCTC NGG 10 8 17 8 24 0 100 5 10
    240605215539_bwsj AGACAGCATCTGTACATCTTATGTGTAAAAAG(+CAGTGT)GATCCAGGGGAAGGTTGTGAGCCACATTGTG NGG 10 8 17 8 24 0 100 5 10
    240605215536_hzyg GCTCTCACAATGTGGCTCACAACCTTCCCCTG(+CAGTGT)GATCCTTTTTACACATAAGATGTACAGATGC NGG 10 8 17 8 24 0 100 5 10
    240605215535_8nzh CTGGTACCCGTGAAGGTCAGAAGAGGGTGAAG(+CAGTGT)GATCCTCTGGAACTGGAGTTACCGATGGTTA NGG 10 8 17 8 24 0 100 5 10
    240605215533_ydzn GTTTATAACCATCGGTAACTCCAGTTCCAGAG(+CAGTGT)GATCCTTCACCCTCTTCTGACCTTCACGGGT NGG 10 8 17 8 24 0 100 5 10
    240605215531_boht ACACACAGCAAACCCCACGGCTTCCCAGACGG(+CAGTGT)GATCCTTCTGCTCCCTTCTACGAGGGCAGAC NGG 10 8 17 8 24 0 100 5 10
    240605215530_a4a2 AGGTAGTCTGCCCTCGTAGAAGGGAGCAGAAG(+CAGTGT)GATCCCGTCTGGGAAGCCGTGGGGTTTGCTG NGG 10 8 17 8 24 0 100 5 10