Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215602_1edf TATTTTAATATGTTATACATCAGTGTATGTAG(+CAGTGT)GATCCAGAGCTTTCCTTCTGAACCTCACTTC NGG 10 8 17 8 24 0 100 5 10
    240605215600_15ag TTTCTGAAGTGAGGTTCAGAAGGAAAGCTCTG(+CAGTGT)GATCCTACATACACTGATGTATAACATATTA NGG 10 8 17 8 24 0 100 5 10
    240605215559_omnm AGATCAGGCCCAGTAGTCTCTAAGAGAGTGAG(+CAGTGT)GATCCTGAGGGAGACTATCCAGCTGCAGGGC NGG 10 8 17 8 24 0 100 5 10
    240605215557_j45d GATCTGCCCTGCAGCTGGATAGTCTCCCTCAG(+CAGTGT)GATCCTCACTCTCTTAGAGACTACTGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240605215556_1zo5 GTGTGTGTGTGTGTGTATGTGTTTCATGTGTG(+CAGTGT)GATCCAAGGGTCGGCCAGGAGCTTAAGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605215553_942s TTACCCTGCCTTAAGCTCCTGGCCGACCCTTG(+CAGTGT)GATCCACACATGAAACACATACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240605215551_awic ACCCGCAAGCTCCAGAAACGGCCCCACCTGGG(+CAGTGT)GATCCATCCCATTTACAGTTACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605215549_gdg1 AGTGTCTGGGTTTGGTAACTGTAAATGGGATG(+CAGTGT)GATCCCCAGGTGGGGCCGTTTCTGGAGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605215547_9ut4 AGACCACCTGCTAAAAGGCTCAGAGACAGAGG(+CAGTGT)GATCCTGGCTCTTTTATACAACTAAGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605215546_e3s1 GTGTCTTTTTCTTAGTTGTATAAAAGAGCCAG(+CAGTGT)GATCCCTCTGTCTCTGAGCCTTTTAGCAGGT NGG 10 8 17 8 24 0 100 5 10