Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215705_k3yw ACAAAACCAGTTTTATGGTGGTGGTGGTGGGG(+CAGTGT)GTACCACCACCACATGATTATATATTGATGG NGG 10 8 17 8 24 0 100 5 10
    240605215703_ab0i ATGAGCCATCAATATATAATCATGTGGTGGTG(+CAGTGT)GTACCCCCACCACCACCACCATAAAACTGGT NGG 10 8 17 8 24 0 100 5 10
    240605215702_yz89 CGGCTGGTGAATGAAGAGACTAACCTATTTGG(+CAGTGT)GTACCTATCATGTGTAAAGCCTGGATACATT NGG 10 8 17 8 24 0 100 5 10
    240605215700_u450 ATGTGAATGTATCCAGGCTTTACACATGATAG(+CAGTGT)GTACCCAAATAGGTTAGTCTCTTCATTCACC NGG 10 8 17 8 24 0 100 5 10
    240605215659_lkuq CCCTCTGTGCATCTGCACTACCTGTGTGTCTG(+CAGTGT)GTACCCGTGAAGGTCAGAAGAGGGTGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605215658_8tns GAGGATCCTTCACCCTCTTCTGACCTTCACGG(+CAGTGT)GTACCAGACACACAGGTAGTGCAGATGCACA NGG 10 8 17 8 24 0 100 5 10
    240605215656_g7uk ATTAGCAGTAACACCTCCCAGATTGAAGTTCG(+CAGTGT)GTACCCTCTGGACTGTTGTAAATCACCAGCC NGG 10 8 17 8 24 0 100 5 10
    240605215655_lkou TCAGGGGCTGGTGATTTACAACAGTCCAGAGG(+CAGTGT)GTACCGAACTTCAATCTGGGAGGTGTTACTG NGG 10 8 17 8 24 0 100 5 10
    240605215652_u4jc GATTCAGGGTGTCTGGTCCATTGTGGGCCCGG(+CAGTGT)GTACCAGAGGTTTTCCACGAAGATCTCTGGC NGG 10 8 17 8 24 0 100 5 10
    240605215650_9xkb ACCTGGCCAGAGATCTTCGTGGAAAACCTCTG(+CAGTGT)GTACCCGGGCCCACAATGGACCAGACACCCT NGG 10 8 17 8 24 0 100 5 10