Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215810_usa1 AAGGGTCAGCTTCTATCTGTAACAGCAATCAG(+CAGTGT)GATCCAGGGGCAAATTCATTCTGAAACAGAA NGG 10 8 17 8 24 0 100 5 10
    240605215807_i4ho ACCGATTCTGTTTCAGAATGAATTTGCCCCTG(+CAGTGT)GATCCTGATTGCTGTTACAGATAGAAGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605215805_i26f GACATTATGGAGGCAACACCAGAAAGCAGAGG(+CAGTGT)GTACCGACATATTGACAGGAAAAAAAAAATA NGG 10 8 17 8 24 0 100 5 10
    240605215804_mnh2 TTTATTATTTTTTTTTTCCTGTCAATATGTCG(+CAGTGT)GTACCCTCTGCTTTCTGGTGTTGCCTCCATA NGG 10 8 17 8 24 0 100 5 10
    240605215803_el7t CAGAATTTGGCATTCAGGAGCACTTCCATATG(+CAGTGT)GTACCCAGGGGCTAGTCATCCAGTAGGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605215800_1zjz GAAGTCCCACCTACTGGATGACTAGCCCCTGG(+CAGTGT)GTACCATATGGAAGTGCTCCTGAATGCCAAA NGG 10 8 17 8 24 0 100 5 10
    240605215757_itgq TATATTCAGCCCTGCAAAAGAAGTCTTCCCCG(+CAGTGT)GATCCCTTAGGCACTTCAGTCACCAGTGTAA NGG 10 8 17 8 24 0 100 5 10
    240605215755_7lxd ATGGGTTACACTGGTGACTGAAGTGCCTAAGG(+CAGTGT)GATCCGGGGAAGACTTCTTTTGCAGGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605215753_8pww GCAGAAGAGATGAGTCCATTCCTCAGGGACAG(+CAGTGT)GTACCTGTACCTACCCCGATTCCAAACCTAC NGG 10 8 17 8 24 0 100 5 10
    240605215752_cipq GGCAGGTAGGTTTGGAATCGGGGTAGGTACAG(+CAGTGT)GTACCTGTCCCTGAGGAATGGACTCATCTCT NGG 10 8 17 8 24 0 100 5 10