Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215913_8gfo TGCTCCCATCCCATCCCCGTGTGCGGCATGGG(+CAGTGT)GATCCAATGCTGCCTCTCCAGCTGAGAGGAT NGG 10 8 17 8 24 0 100 5 10
    240605215912_khh5 CTAGCATCCTCTCAGCTGGAGAGGCAGCATTG(+CAGTGT)GATCCCCATGCCGCACACGGGGATGGGATGG NGG 10 8 17 8 24 0 100 5 10
    240605215911_lhvf TTCCATACCCTTTCTTCTGTGTTAGCCTGCAG(+CAGTGT)GATCCCTTAAGTACCCTTATTCCAATAGGCC NGG 10 8 17 8 24 0 100 5 10
    240605215909_6o84 ACCCTGGCCTATTGGAATAAGGGTACTTAAGG(+CAGTGT)GATCCTGCAGGCTAACACAGAAGAAAGGGTA NGG 10 8 17 8 24 0 100 5 10
    240605215908_ch1m CCGTGGAGCTTTAACACGGAGAATCGAGTTTG(+CAGTGT)GATCCCAGAGACATCAGGCAGCCTTGAGACC NGG 10 8 17 8 24 0 100 5 10
    240605215906_vsra TGCCAGGTCTCAAGGCTGCCTGATGTCTCTGG(+CAGTGT)GATCCAAACTCGATTCTCCGTGTTAAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240605215904_m7nb CTTGTTCTAATCCTAACCCTAACCCAATTCGG(+CAGTGT)GATCCCAGCTGGTGCTCTTAAACACACACAG NGG 10 8 17 8 24 0 100 5 10
    240605215902_w2p1 AACAACTGTGTGTGTTTAAGAGCACCAGCTGG(+CAGTGT)GATCCCGAATTGGGTTAGGGTTAGGATTAGA NGG 10 8 17 8 24 0 100 5 10
    240605215859_ncar GAAGACAGCCGCTTAGGGAGGGACTACCTTGG(+CAGTGT)GATCCAATGAAACCGGCCAGAGAGAGAGAGA NGG 10 8 17 8 24 0 100 5 10
    240605215858_8ruz CTCTCTCTCTCTCTCTCTGGCCGGTTTCATTG(+CAGTGT)GATCCCAAGGTAGTCCCTCCCTAAGCGGCTG NGG 10 8 17 8 24 0 100 5 10