Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215857_o74f ACCCTACTCTTAGCTGCACCTCTTAGATTCTG(+CAGTGT)GTACCTTTCGTTTTATCACCCAGGTGTTGCT NGG 10 8 17 8 24 0 100 5 10
    240605215856_i5in TGCCCAGCAACACCTGGGTGATAAAACGAAAG(+CAGTGT)GTACCAGAATCTAAGAGGTGCAGCTAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240605215854_yfyz AGTGCCCCTACACCAGCACTGCCAGTTATCTG(+CAGTGT)GATCCCTAGTTACCGGCGCCGGTTTCCTGCC NGG 10 8 17 8 24 0 100 5 10
    240605215852_mznh GTCAAGGCAGGAAACCGGCGCCGGTAACTAGG(+CAGTGT)GATCCAGATAACTGGCAGTGCTGGTGTAGGG NGG 10 8 17 8 24 0 100 5 10
    240605215851_eamj CGACCTGTTCACTGTGAGCTCCATCTTGATCG(+CAGTGT)GTACCAGAGGTCAGATTGGGTTTAAGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605215849_kbpe CGAGGAAAGCCTTAAACCCAATCTGACCTCTG(+CAGTGT)GTACCGATCAAGATGGAGCTCACAGTGAACA NGG 10 8 17 8 24 0 100 5 10
    240605215847_gt67 CAGGGTCCACCTTCCTCTTCTGTCCTTTGCAG(+CAGTGT)GTACCAGACACCCTTATGGTGCACAGTCATA NGG 10 8 17 8 24 0 100 5 10
    240605215845_to9q ATATGTATGACTGTGCACCATAAGGGTGTCTG(+CAGTGT)GTACCTGCAAAGGACAGAAGAGGAAGGTGGA NGG 10 8 17 8 24 0 100 5 10
    240605215844_a9nz CCTTTGCAGATGAAGGGCAGAGTCAGTGCTGG(+CAGTGT)GATCCCAGCCCTGCCCTCTATTACAGCATAC NGG 10 8 17 8 24 0 100 5 10
    240605215842_oj6m CCTGTGTATGCTGTAATAGAGGGCAGGGCTGG(+CAGTGT)GATCCCAGCACTGACTCTGCCCTTCATCTGC NGG 10 8 17 8 24 0 100 5 10