Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215935_7ils AAAATATATCGGCAGTCACTTCTTTTATAAAG(+CAGTGT)GTACCATGGGCTACATTACACAATAGTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605215932_u1wj TACATTGAGACTATTGTGTAATGTAGCCCATG(+CAGTGT)GTACCTTTATAAAAGAAGTGACTGCCGATAT NGG 10 8 17 8 24 0 100 5 10
    240605215931_1aue GTTTGATACCCAGGCCTTGCTCTGCTTAGTAG(+CAGTGT)GTACCATGGGCCGTTTTAAGAGCTACACATG NGG 10 8 17 8 24 0 100 5 10
    240605215928_021x TCTGCCATGTGTAGCTCTTAAAACGGCCCATG(+CAGTGT)GTACCTACTAAGCAGAGCAAGGCCTGGGTAT NGG 10 8 17 8 24 0 100 5 10
    240605215926_qhye CACTGAGTCAGGGCTCCGGCTTCTGGCTGTGG(+CAGTGT)GTACCCACCGCCCAGCTGCAAGAGGGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240605215924_syu3 ATTGGTGACTCCCTCTTGCAGCTGGGCGGTGG(+CAGTGT)GTACCCACAGCCAGAAGCCGGAGCCCTGACT NGG 10 8 17 8 24 0 100 5 10
    240605215922_97gx TTTAAGTCCCCAGGGTAGCTGGGGGCAGTTAG(+CAGTGT)GTACCCCTTGCTCCCCCAGCCCTCCCCACCT NGG 10 8 17 8 24 0 100 5 10
    240605215920_8yjr GGCCTAGGTGGGGAGGGCTGGGGGAGCAAGGG(+CAGTGT)GTACCTAACTGCCCCCAGCTACCCTGGGGAC NGG 10 8 17 8 24 0 100 5 10
    240605215918_fxpi TTGGGACCCAATTCCCCTTTCTCCAAGACAGG(+CAGTGT)GATCCAGCGGCCCAGGATGCCCACTGGGGTC NGG 10 8 17 8 24 0 100 5 10
    240605215916_kip1 AGCAGGACCCCAGTGGGCATCCTGGGCCGCTG(+CAGTGT)GATCCCTGTCTTGGAGAAAGGGGAATTGGGT NGG 10 8 17 8 24 0 100 5 10