Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605220007_a477 CAGAGATGTCTGTCAAACATGTATATGCAGCG(+CAGTGT)GATCCATCTGCTTGTTTGGTTGGGTTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605220005_wr31 CAACAAAAACAACCCAACCAAACAAGCAGATG(+CAGTGT)GATCCGCTGCATATACATGTTTGACAGACAT NGG 10 8 17 8 24 0 100 5 10
    240605220003_frkz GTTGTTAGAGCAAAGACACCATTCCATTCCAG(+CAGTGT)GTACCAAGTGAAGCATACTTGACAAATAGGC NGG 10 8 17 8 24 0 100 5 10
    240605220002_gtnk AGACTGCCTATTTGTCAAGTATGCTTCACTTG(+CAGTGT)GTACCTGGAATGGAATGGTGTCTTTGCTCTA NGG 10 8 17 8 24 0 100 5 10
    240605220000_1c1o TGCAGAGATGGTTTATGAGGCGGGTCCGAAGG(+CAGTGT)GTACCCTTCACCTTGCCTGGAGATATACAAA NGG 10 8 17 8 24 0 100 5 10
    240605215958_f8wg CAGGTTTTGTATATCTCCAGGCAAGGTGAAGG(+CAGTGT)GTACCCTTCGGACCCGCCTCATAAACCATCT NGG 10 8 17 8 24 0 100 5 10
    240605215957_g3sy TCCAATCTCGGAAAACAAATCAAAATATTAAG(+CAGTGT)GATCCCTGAAATATTAATCCTAATGAGATCT NGG 10 8 17 8 24 0 100 5 10
    240605215955_qw9i CAGTGAGATCTCATTAGGATTAATATTTCAGG(+CAGTGT)GATCCTTAATATTTTGATTTGTTTTCCGAGA NGG 10 8 17 8 24 0 100 5 10
    240605215954_tk3z TCTGTGCTCAGGTCACACAGGGGGCTTCAGAG(+CAGTGT)GATCCTTATGAAAGATGAGTTAAAAAATGTC NGG 10 8 17 8 24 0 100 5 10
    240605215953_m741 CACTTGACATTTTTTAACTCATCTTTCATAAG(+CAGTGT)GATCCTCTGAAGCCCCCTGTGTGACCTGAGC NGG 10 8 17 8 24 0 100 5 10