Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215826_l90c TCTGGAGAAACTCACGACAGAGGTGGTGCTTG(+CAGTGT)GTACCTAGTTCCCAGGGCCAGGTAAACTTGT NGG 10 8 17 8 24 0 100 5 10
    240605215825_0k4q GTGACACAAGTTTACCTGGCCCTGGGAACTAG(+CAGTGT)GTACCAAGCACCACCTCTGTCGTGAGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605215823_cww5 CCACTGCTGCGACACGTGGAGGCCCCACAGTG(+CAGTGT)GTACCCCAGAGTGGGAAGGTTGGATGGCTCA NGG 10 8 17 8 24 0 100 5 10
    240605215821_xkz4 TCCCATGAGCCATCCAACCTTCCCACTCTGGG(+CAGTGT)GTACCACTGTGGGGCCTCCACGTGTCGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605215820_8gep GAGGTCCATTCTCACTGGTTGGGGTCCATGTG(+CAGTGT)GTACCAGTTCCCAAGTATTTACTCTACACAG NGG 10 8 17 8 24 0 100 5 10
    240605215819_l0wb TCTCTCTGTGTAGAGTAAATACTTGGGAACTG(+CAGTGT)GTACCACATGGACCCCAACCAGTGAGAATGG NGG 10 8 17 8 24 0 100 5 10
    240605215816_t22b TCGCTCGATCAGCTTGTTTTTGTACGCCCCAG(+CAGTGT)GATCCTATGCTCAGGGGAGGTGTAGCCCACA NGG 10 8 17 8 24 0 100 5 10
    240605215815_pqm4 GTGGCTGTGGGCTACACCTCCCCTGAGCATAG(+CAGTGT)GATCCTGGGGCGTACAAAAACAAGCTGATCG NGG 10 8 17 8 24 0 100 5 10
    240605215813_bp32 GTCCTCTGGCTTCTGCTACACCATCAATCCTG(+CAGTGT)GATCCTCACCAGGACTTCTCTCAGTTATCCT NGG 10 8 17 8 24 0 100 5 10
    240605215811_r69r ACAAGAGGATAACTGAGAGAAGTCCTGGTGAG(+CAGTGT)GATCCAGGATTGATGGTGTAGCAGAAGCCAG NGG 10 8 17 8 24 0 100 5 10