Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215751_9jre TGAAAGAGCTCACACTTCCGAGGCGGAAATGG(+CAGTGT)GTACCAAAATGAGAGATCAGCAAGATTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605215749_hdbq ATCTTGGGAAATCTTGCTGATCTCTCATTTTG(+CAGTGT)GTACCCATTTCCGCCTCGGAAGTGTGAGCTC NGG 10 8 17 8 24 0 100 5 10
    240605215747_t8pl TTTCTGTAGGAAATGAAATAACTGATTTTGAG(+CAGTGT)GTACCAAATAGTGCAATTGGGTAAGACAGGG NGG 10 8 17 8 24 0 100 5 10
    240605215746_5usf ATAAACCCTGTCTTACCCAATTGCACTATTTG(+CAGTGT)GTACCTCAAAATCAGTTATTTCATTTCCTAC NGG 10 8 17 8 24 0 100 5 10
    240605215744_ioz6 GCAGGCATCCTGAGAGGTGCCATGACAACCAG(+CAGTGT)GATCCTGTTCACAGCCGATCAGTGTGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605215743_bmp4 GAAGTCTCTCCACACTGATCGGCTGTGAACAG(+CAGTGT)GATCCTGGTTGTCATGGCACCTCTCAGGATG NGG 10 8 17 8 24 0 100 5 10
    240605215742_126b TGAATATAAGGGATCTTACTGTCATTTGTTAG(+CAGTGT)GTACCACTGAGTAATTCCATAGTTTCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605215741_57xv AATTACCACAGAAACTATGGAATTACTCAGTG(+CAGTGT)GTACCTAACAAATGACAGTAAGATCCCTTAT NGG 10 8 17 8 24 0 100 5 10
    240605215738_j899 TGCCTGCAGTTTTAACTTTATAGCTCTCCCAG(+CAGTGT)GTACCAGCTTTGGGGCCAGCACACGGCACTC NGG 10 8 17 8 24 0 100 5 10
    240605215736_q7r6 AATGAGAGTGCCGTGTGCTGGCCCCAAAGCTG(+CAGTGT)GTACCTGGGAGAGCTATAAAGTTAAAACTGC NGG 10 8 17 8 24 0 100 5 10