Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215722_f22l CTCCTGGAAGAGAAAGGTCATAGAACACATAG(+CAGTGT)GTACCCCACCCCTCTGGAAGGCAGAGGGTAA NGG 10 8 17 8 24 0 100 5 10
    240605215720_79v6 TGTAATTACCCTCTGCCTTCCAGAGGGGTGGG(+CAGTGT)GTACCTATGTGTTCTATGACCTTTCTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240605215718_6839 CATCCAATTCATTTGAATATCTTATATTGTGG(+CAGTGT)GTACCTTTTTTAAATAGAAAACTTAAGATGA NGG 10 8 17 8 24 0 100 5 10
    240605215717_vee1 AAGAATCATCTTAAGTTTTCTATTTAAAAAAG(+CAGTGT)GTACCCACAATATAAGATATTCAAATGAATT NGG 10 8 17 8 24 0 100 5 10
    240605215715_8ay6 TTCATAGGTGAAATCTTGCCTCCCAGTGTAAG(+CAGTGT)GTACCTGGTTTCTTTTTGTGATACTTCTCTT NGG 10 8 17 8 24 0 100 5 10
    240605215713_b5nv AGTTAAAGAGAAGTATCACAAAAAGAAACCAG(+CAGTGT)GTACCTTACACTGGGAGGCAAGATTTCACCT NGG 10 8 17 8 24 0 100 5 10
    240605215711_lwgd AGCAACAGAAAAACAACCAAGATGTTGTGGTG(+CAGTGT)GTACCTTCCTAAAATCCCGGCACTTGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240605215710_eqnk CTCCACCTTCCAAGTGCCGGGATTTTAGGAAG(+CAGTGT)GTACCACCACAACATCTTGGTTGTTTTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605215708_nzcy TGGTTGCCTTCCTCCCTCGGAATGCTTGCACG(+CAGTGT)GTACCTTCTAGAACCATGAACGTTAACCCCT NGG 10 8 17 8 24 0 100 5 10
    240605215707_9pvg TCCCCAGGGGTTAACGTTCATGGTTCTAGAAG(+CAGTGT)GTACCGTGCAAGCATTCCGAGGGAGGAAGGC NGG 10 8 17 8 24 0 100 5 10