Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215634_yfpr ACCTACAGAGCACAGGGCACCTCAGCTATTGG(+CAGTGT)GTACCGGAACACAAACACCAGTAACTCCTCA NGG 10 8 17 8 24 0 100 5 10
    240605215632_ds23 CCATGTGAGGAGTTACTGGTGTTTGTGTTCCG(+CAGTGT)GTACCCAATAGCTGAGGTGCCCTGTGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240605215631_oeq4 GAAAGAAAACCACATGTCTTGCCTCATCTGTG(+CAGTGT)GATCCTGGCCTATAATGTGCACATGTAGACT NGG 10 8 17 8 24 0 100 5 10
    240605215629_l5yr CCCTGAGTCTACATGTGCACATTATAGGCCAG(+CAGTGT)GATCCACAGATGAGGCAAGACATGTGGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605215627_cey9 TGCAGGCACCAGGCACGAATGTGGAGAGCATG(+CAGTGT)GATCCGTACAGGCACTCCCATATGCACAAAA NGG 10 8 17 8 24 0 100 5 10
    240605215626_7omv TTATTTTTTGTGCATATGGGAGTGCCTGTACG(+CAGTGT)GATCCATGCTCTCCACATTCGTGCCTGGTGC NGG 10 8 17 8 24 0 100 5 10
    240605215625_gbcn GATTCCTGATTCCTTCTCAACATGTGTGTAAG(+CAGTGT)GATCCTCGACCTGCAAAGTCCATCTCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605215624_4q8n TTCATGGAAAGAGATGGACTTTGCAGGTCGAG(+CAGTGT)GATCCTTACACACATGTTGAGAAGGAATCAG NGG 10 8 17 8 24 0 100 5 10
    240605215622_1dbw GCCAACACCAAACGAATCAGTAGCAGCTGCTG(+CAGTGT)GATCCAGCAGAAACCACAAGGCTCCACCGAA NGG 10 8 17 8 24 0 100 5 10
    240605215621_fff6 GCAGATTCGGTGGAGCCTTGTGGTTTCTGCTG(+CAGTGT)GATCCAGCAGCTGCTACTGATTCGTTTGGTG NGG 10 8 17 8 24 0 100 5 10