Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215529_mgt5 ATGCTTTCTAAGGATGCTCGATAAACTTATTG(+CAGTGT)GATCCCCAGAGAGCACGTTGGCAGAATCATG NGG 10 8 17 8 24 0 100 5 10
    240605215527_byy1 TGAAGCATGATTCTGCCAACGTGCTCTCTGGG(+CAGTGT)GATCCAATAAGTTTATCGAGCATCCTTAGAA NGG 10 8 17 8 24 0 100 5 10
    240605215526_30kb ACCTGGAATTTGCCTATGCTCAGTCTTGGATG(+CAGTGT)GATCCGGAATAGTTTCATGGAACAGTGATAA NGG 10 8 17 8 24 0 100 5 10
    240605215524_k5ah GGCAATTATCACTGTTCCATGAAACTATTCCG(+CAGTGT)GATCCATCCAAGACTGAGCATAGGCAAATTC NGG 10 8 17 8 24 0 100 5 10
    240605215522_pqk9 CAGATCTTCCCACCTCAGGCTCCAAGTGCTGG(+CAGTGT)GATCCCAACCCTAACCCTAAGGGACAGATTT NGG 10 8 17 8 24 0 100 5 10
    240605215520_oich GACATAAATCTGTCCCTTAGGGTTAGGGTTGG(+CAGTGT)GATCCCAGCACTTGGAGCCTGAGGTGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605215519_axg1 GTCTATTTGATGAGAGAATAACTGGGCACCAG(+CAGTGT)GATCCTATTTTGGAAGCTTCACTTATGTACC NGG 10 8 17 8 24 0 100 5 10
    240605215517_eff9 CATATGGTACATAAGTGAAGCTTCCAAAATAG(+CAGTGT)GATCCTGGTGCCCAGTTATTCTCTCATCAAA NGG 10 8 17 8 24 0 100 5 10
    240605215515_rmzf GCAAGTGCTCATGACAGTTGCGGGGAAGACCG(+CAGTGT)GATCCGGAGGTGTTACAAGCTTTGATAAATG NGG 10 8 17 8 24 0 100 5 10
    240605215513_u9m9 CTGGCCATTTATCAAAGCTTGTAACACCTCCG(+CAGTGT)GATCCGGTCTTCCCCGCAACTGTCATGAGCA NGG 10 8 17 8 24 0 100 5 10