Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605215201_zb6i CCACCATTGCTTCTGTAACTGCTGGGCGCTGG(+CAGTGT)GATCCTGCCCCCAGGAAGTTAAGAGTGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605215200_rvtk GCTCCTGCCCACTCTTAACTTCCTGGGGGCAG(+CAGTGT)GATCCCAGCGCCCAGCAGTTACAGAAGCAAT NGG 10 8 17 8 24 0 100 5 10
    240605215158_7orj CCTTTTTTAAAGATTTATTATTTTTGAGATAG(+CAGTGT)GTACCTCTGGATGGCCTGGAACTTGCCATGT NGG 10 8 17 8 24 0 100 5 10
    240605215156_a193 GGTCTACATGGCAAGTTCCAGGCCATCCAGAG(+CAGTGT)GTACCTATCTCAAAAATAATAAATCTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240605215155_oo81 AGCTTTGAAACTGGGCAAGATCCAGTTCATAG(+CAGTGT)GATCCCTTCTCGGGACCATGTCTCAGTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605215153_edzk GGTGCAGGAACTGAGACATGGTCCCGAGAAGG(+CAGTGT)GATCCTATGAACTGGATCTTGCCCAGTTTCA NGG 10 8 17 8 24 0 100 5 10
    240605215152_pk1g GTCGGACTGAAGCCCCCTGAACAAGGGATGAG(+CAGTGT)GTACCAGGGAGAGCTTCCTCGCTGGACGGGA NGG 10 8 17 8 24 0 100 5 10
    240605215150_q6yv GTGGGTCCCGTCCAGCGAGGAAGCTCTCCCTG(+CAGTGT)GTACCTCATCCCTTGTTCAGGGGGCTTCAGT NGG 10 8 17 8 24 0 100 5 10
    240605215148_xasq CAGAAGATGCTAAGCTGTCATAGGTGGAGCAG(+CAGTGT)GATCCTAAGGACGCACAATATCAGTTGTGCG NGG 10 8 17 8 24 0 100 5 10
    240605215146_hc4l CAGCACGCACAACTGATATTGTGCGTCCTTAG(+CAGTGT)GATCCTGCTCCACCTATGACAGCTTAGCATC NGG 10 8 17 8 24 0 100 5 10