Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605214931_b83j CAGTCACCCTTGGTGACATCACACTAACACGG(+CAGTGT)GTACCAGAAGAGCAAGTCTGCCCGGCAGAGC NGG 10 8 17 8 24 0 100 5 10
    240605214930_o3a3 ACTGGGCTCTGCCGGGCAGACTTGCTCTTCTG(+CAGTGT)GTACCCGTGTTAGTGTGATGTCACCAAGGGT NGG 10 8 17 8 24 0 100 5 10
    240605214928_skyr CTCCTAGCAAACTTGGAGACCCCCCCAGTCTG(+CAGTGT)GATCCAACTGTAACTCTCTGTGCTCCTGGTT NGG 10 8 17 8 24 0 100 5 10
    240605214926_mwqm TCCAGAACCAGGAGCACAGAGAGTTACAGTTG(+CAGTGT)GATCCAGACTGGGGGGGTCTCCAAGTTTGCT NGG 10 8 17 8 24 0 100 5 10
    240605214925_9om2 GCCCTTCAGTCTGCTCCTCCACGGGGTGTTCG(+CAGTGT)GATCCCGATTGAGGCCCTGAATGCCCGCCAT NGG 10 8 17 8 24 0 100 5 10
    240605214924_r4y9 GAGGAATGGCGGGCATTCAGGGCCTCAATCGG(+CAGTGT)GATCCGAACACCCCGTGGAGGAGCAGACTGA NGG 10 8 17 8 24 0 100 5 10
    240605214922_93cd TAGCCCCCAGCATCACAGAAAATGACCTAGTG(+CAGTGT)GTACCCACCTGTGATCCCAGGCACCAGAAGA NGG 10 8 17 8 24 0 100 5 10
    240605214921_0vnp CCAGATCTTCTGGTGCCTGGGATCACAGGTGG(+CAGTGT)GTACCACTAGGTCATTTTCTGTGATGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605214919_2g5f TGCAGGTGGGGCCCACTAGATGGTTTCTGGGG(+CAGTGT)GATCCCCAGAACATGGGGTTCACCTATCCTA NGG 10 8 17 8 24 0 100 5 10
    240605214917_nsej GTAGATAGGATAGGTGAACCCCATGTTCTGGG(+CAGTGT)GATCCCCCAGAAACCATCTAGTGGGCCCCAC NGG 10 8 17 8 24 0 100 5 10