Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605214805_0g6u GTGGGACAGGCCATGATTAGGGGCACAGGGAG(+CAGTGT)GATCCCAGGGCCTTAATGGAGTCAACCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605214802_w849 TGTCTCCCAGGTTGACTCCATTAAGGCCCTGG(+CAGTGT)GATCCTCCCTGTGCCCCTAATCATGGCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605214800_21zh GTTTGTTCCTCTTGTCAAGGCTTGCTCTCTGG(+CAGTGT)GATCCCCTCTGCCCCAGGACAAGGGGACCTT NGG 10 8 17 8 24 0 100 5 10
    240605214758_oaej AGGGGAAGGTCCCCTTGTCCTGGGGCAGAGGG(+CAGTGT)GATCCCAGAGAGCAAGCCTTGACAAGAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605214756_s9gf ACAGCGGGTGAGAGGTGTCTCTGTGTCTCCGG(+CAGTGT)GTACCATAGGCCCTACGAGGTTGCCAGCACC NGG 10 8 17 8 24 0 100 5 10
    240605214754_d6eu GAGGCGGTGCTGGCAACCTCGTAGGGCCTATG(+CAGTGT)GTACCCGGAGACACAGAGACACCTCTCACCC NGG 10 8 17 8 24 0 100 5 10
    240605214753_r2np CAGGCTTCCCCATGAGCTCTGGGCCCTCCAAG(+CAGTGT)GATCCACATCCCCCAACAGCCAGTTCCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605214751_r6e9 TCTGTCACTGGAACTGGCTGTTGGGGGATGTG(+CAGTGT)GATCCTTGGAGGGCCCAGAGCTCATGGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605214749_sezw GAGTAAAGTTAGACCAGGACAGAGGGCCATGG(+CAGTGT)GTACCTAGGTAGCCTCAGTTTCCCCTCTCTG NGG 10 8 17 8 24 0 100 5 10
    240605214747_a00q AGTGACAGAGAGGGGAAACTGAGGCTACCTAG(+CAGTGT)GTACCCATGGCCCTCTGTCCTGGTCTAACTT NGG 10 8 17 8 24 0 100 5 10