Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605214527_9kun AGAATCGCTTAAGCAGGGCTGTGGGCCTGCAG(+CAGTGT)GATCCCCAGGTCCCTTTGGACCGGAGGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605214524_l3yq CGTCTTCCCCCTCCGGTCCAAAGGGACCTGGG(+CAGTGT)GATCCTGCAGGCCCACAGCCCTGCTTAAGCG NGG 10 8 17 8 24 0 100 5 10
    240605214522_h35s TTGTGGGTCATGAATTCTCTGTACCCCTGCTG(+CAGTGT)GATCCTTCTAATTAAATTTGTTTTTCCTTCG NGG 10 8 17 8 24 0 100 5 10
    240605214521_cfa9 GGTGACGAAGGAAAAACAAATTTAATTAGAAG(+CAGTGT)GATCCAGCAGGGGTACAGAGAATTCATGACC NGG 10 8 17 8 24 0 100 5 10
    240605214519_i38v CTGTGAAGTCTATTGTGGCCACAGACTGCTGG(+CAGTGT)GTACCAAGTCTCAGGAGAACCCAGCCTAGAT NGG 10 8 17 8 24 0 100 5 10
    240605214518_2z1q CCTAAATCTAGGCTGGGTTCTCCTGAGACTTG(+CAGTGT)GTACCCAGCAGTCTGTGGCCACAATAGACTT NGG 10 8 17 8 24 0 100 5 10
    240605214516_2oce GATAATCTATCATTTGAATGGCAAGAGACCTG(+CAGTGT)GTACCCTCTATCTCGCTACTCTGGAGCCTCC NGG 10 8 17 8 24 0 100 5 10
    240605214514_ogz4 CTAGGGGAGGCTCCAGAGTAGCGAGATAGAGG(+CAGTGT)GTACCAGGTCTCTTGCCATTCAAATGATAGA NGG 10 8 17 8 24 0 100 5 10
    240605214512_olrp TCAACTCAGGCTCCCCATGACACTGGACCTGG(+CAGTGT)GTACCACAAGAAACCGGGTGAGGCCTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240605214511_euw5 TTCCTCTCCCAGGCCTCACCCGGTTTCTTGTG(+CAGTGT)GTACCCAGGTCCAGTGTCATGGGGAGCCTGA NGG 10 8 17 8 24 0 100 5 10