Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605214451_u4f0 TTAGTCTGGTACAATCCTTTCTAGAAGAAAGG(+CAGTGT)GATCCCTAAAGTGAAGGAATAGATTCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240605214450_q27e GATGGGAGGGGAATCTATTCCTTCACTTTAGG(+CAGTGT)GATCCCTTTCTTCTAGAAAGGATTGTACCAG NGG 10 8 17 8 24 0 100 5 10
    240605214447_sroy GCTTCCTGAACCTCACTCTCACTTCCTAGGGG(+CAGTGT)GATCCTGGAAGCTGGGTACGAACTAGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605214445_mw2y GTCTCCTTCCCTAGTTCGTACCCAGCTTCCAG(+CAGTGT)GATCCCCCTAGGAAGTGAGAGTGAGGTTCAG NGG 10 8 17 8 24 0 100 5 10
    240605214444_ujzt TGCACTCAGGAGAGGGAGAACCTCCCTGGATG(+CAGTGT)GTACCCAGATGAAGTCAGAGCCGTGCTCTTG NGG 10 8 17 8 24 0 100 5 10
    240605214442_xpnl GGCAACAAGAGCACGGCTCTGACTTCATCTGG(+CAGTGT)GTACCATCCAGGGAGGTTCTCCCTCTCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605214440_05hw CTGGGAAGCAGAGGCAAGCTCAGGATACAAGG(+CAGTGT)GTACCTTCCCCTTGCCTGGGTCTTCCCTCGA NGG 10 8 17 8 24 0 100 5 10
    240605214438_fllt TAGGCTCGAGGGAAGACCCAGGCAAGGGGAAG(+CAGTGT)GTACCCTTGTATCCTGAGCTTGCCTCTGCTT NGG 10 8 17 8 24 0 100 5 10
    240605214436_rlx0 ATCAGCTGCAGCCTGGCTGCCTTATCTCCCTG(+CAGTGT)GTACCACTGGCTTCTGCAACCCATGGGGTTA NGG 10 8 17 8 24 0 100 5 10
    240605214434_ho9g GCCATTAACCCCATGGGTTGCAGAAGCCAGTG(+CAGTGT)GTACCAGGGAGATAAGGCAGCCAGGCTGCAG NGG 10 8 17 8 24 0 100 5 10