Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605214345_h3x5 TGCACATGCCCTTTTCTGTCCAGTTTAGGTTG(+CAGTGT)GATCCTTAGTACCTAGACACACTCTCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240605214344_xtge CACGTAAGAGGAGAGTGTGTCTAGGTACTAAG(+CAGTGT)GATCCAACCTAAACTGGACAGAAAAGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240605214343_bw6m TGTGTCCCCAATCCCATCCATCTGTCTGAAAG(+CAGTGT)GATCCCAGCATCCCTAGGCTTGGCATCTGAG NGG 10 8 17 8 24 0 100 5 10
    240605214341_y24m CCTTCCTCAGATGCCAAGCCTAGGGATGCTGG(+CAGTGT)GATCCTTTCAGACAGATGGATGGGATTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605214340_lkos AAGTTTAAATAATCTAAGTTTCTTTGCAGCTG(+CAGTGT)GATCCAACTGCTAGAAGGCAATTCTTAGAGC NGG 10 8 17 8 24 0 100 5 10
    240605214338_y6rf AGATTGCTCTAAGAATTGCCTTCTAGCAGTTG(+CAGTGT)GATCCAGCTGCAAAGAAACTTAGATTATTTA NGG 10 8 17 8 24 0 100 5 10
    240605214336_91wz GGTTGAAGGAAGAAGACCTACTCTGAACTTGG(+CAGTGT)GTACCAATATCCCATGGGCAGAACAAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605214335_n3ml TTTATTCTTTTTGTTCTGCCCATGGGATATTG(+CAGTGT)GTACCCAAGTTCAGAGTAGGTCTTCTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240605214334_ydyj CCCTAAACAGGGCAGCAGAATATAGTTTAGTG(+CAGTGT)GTACCCAGCTATAGAAATAAATCTTAGGGGT NGG 10 8 17 8 24 0 100 5 10
    240605214332_8x8q CTCTGACCCCTAAGATTTATTTCTATAGCTGG(+CAGTGT)GTACCACTAAACTATATTCTGCTGCCCTGTT NGG 10 8 17 8 24 0 100 5 10