Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605214432_ko0b GGACTTGCAGACCCAGCACTCACGGGCACTGG(+CAGTGT)GATCCATCCCAGAGAGTGCACCTGAGTAGCT NGG 10 8 17 8 24 0 100 5 10
    240605214429_cxti GGAAGAGCTACTCAGGTGCACTCTCTGGGATG(+CAGTGT)GATCCCAGTGCCCGTGAGTGCTGGGTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240605214427_twno CGGACATGGAATCTTGAAGACCCAAGGCAGGG(+CAGTGT)GATCCCAAGGGCTCCATGCAGAACTCACAGG NGG 10 8 17 8 24 0 100 5 10
    240605214425_qg7i ATCCTCCTGTGAGTTCTGCATGGAGCCCTTGG(+CAGTGT)GATCCCCTGCCTTGGGTCTTCAAGATTCCAT NGG 10 8 17 8 24 0 100 5 10
    240605214424_asob TTATTTTTCTAAATAGTGTTCAGAAAACTCTG(+CAGTGT)GATCCAAAATGTGGCATTTTTCTGAGAATGA NGG 10 8 17 8 24 0 100 5 10
    240605214422_trdm AATCTTCATTCTCAGAAAAATGCCACATTTTG(+CAGTGT)GATCCAGAGTTTTCTGAACACTATTTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605214421_e1p1 ACTAAGCTGATCTGGCTATCCCAATGCATGTG(+CAGTGT)GTACCTGATAGATGGATATGGTAGAGATTAT NGG 10 8 17 8 24 0 100 5 10
    240605214420_3tcx GGGAAATAATCTCTACCATATCCATCTATCAG(+CAGTGT)GTACCACATGCATTGGGATAGCCAGATCAGC NGG 10 8 17 8 24 0 100 5 10
    240605214418_lc1k TTATCATCTCTGACCTTCTGAAGCCAGGGGTG(+CAGTGT)GTACCCATCCCTAGCTCAGTCTAATTAAATC NGG 10 8 17 8 24 0 100 5 10
    240605214416_gf60 GGAAAGATTTAATTAGACTGAGCTAGGGATGG(+CAGTGT)GTACCACCCCTGGCTTCAGAAGGTCAGAGAT NGG 10 8 17 8 24 0 100 5 10