Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605214116_2kqk ACATCTAGAATGTTCATTTTTCCTGCCCCCAG(+CAGTGT)GTACCCTAGTCAGGTGTGGGCTAGCCAGGTG NGG 10 8 17 8 24 0 100 5 10
    240605214115_wvpm TGAATCACCTGGCTAGCCCACACCTGACTAGG(+CAGTGT)GTACCTGGGGGCAGGAAAAATGAACATTCTA NGG 10 8 17 8 24 0 100 5 10
    240605214113_tsa8 CCTCGCTTAGGATGGAGGAGGGGATGCTGTGG(+CAGTGT)GTACCACCCCACCCCAGCAGTACTCCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605214111_4db6 AAGTCCCCAGGGAGTACTGCTGGGGTGGGGTG(+CAGTGT)GTACCCACAGCATCCCCTCCTCCATCCTAAG NGG 10 8 17 8 24 0 100 5 10
    240605214109_bmc1 GCTCTCAGAAAGGTGCCCCTGCTAGCCTTTGG(+CAGTGT)GATCCTCTTGGCTACGGCACCTACTCTCCAA NGG 10 8 17 8 24 0 100 5 10
    240605214108_mwvb GTGGGTTGGAGAGTAGGTGCCGTAGCCAAGAG(+CAGTGT)GATCCCAAAGGCTAGCAGGGGCACCTTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605214106_5ks9 GCTTGTAAACTATGAGGCACTACAGTGTCCAG(+CAGTGT)GATCCCCACGTCGGCCAAGCAGCTCTGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605214105_t07t GACCTGCTGCAGAGCTGCTTGGCCGACGTGGG(+CAGTGT)GATCCTGGACACTGTAGTGCCTCATAGTTTA NGG 10 8 17 8 24 0 100 5 10
    240605214103_nbay GAGCTATGGCCCTGACCTGTGGATAACAAACG(+CAGTGT)GATCCCTCGTGATGTAGCCTGGGCAGTTAGT NGG 10 8 17 8 24 0 100 5 10
    240605214101_xhxl TGTCCACTAACTGCCCAGGCTACATCACGAGG(+CAGTGT)GATCCGTTTGTTATCCACAGGTCAGGGCCAT NGG 10 8 17 8 24 0 100 5 10