Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605214027_ugf8 CTTCTGATTGATGGTACCATCCCCCAAGAAGG(+CAGTGT)GATCCTCTCAGGAAGAAATGCAGGCTTTCTA NGG 10 8 17 8 24 0 100 5 10
    240605214026_y65y GAGTTTAGAAAGCCTGCATTTCTTCCTGAGAG(+CAGTGT)GATCCCTTCTTGGGGGATGGTACCATCAATC NGG 10 8 17 8 24 0 100 5 10
    240605214024_buu2 CCTCTGTAGACCAGGCTGGCCATAAACTCAGG(+CAGTGT)GATCCACCTGCCTCTACTTTCGGAGTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605214022_iafi TAATCTCAGCACTCCGAAAGTAGAGGCAGGTG(+CAGTGT)GATCCCTGAGTTTATGGCCAGCCTGGTCTAC NGG 10 8 17 8 24 0 100 5 10
    240605214021_t2ys GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+CAGTGT)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605214018_g3il GGATGGACCATGTAGAGACTGCCATATCCAGG(+CAGTGT)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240605214017_7yuw TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+CAGTGT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605214015_u1jt TCCTCAGAAAATTGGACATAGTACTACCGGAG(+CAGTGT)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240605214013_wr90 GTGTCCTGGATTACCTGGATGTTTTGAGTTAG(+CAGTGT)GATCCTTTTGCATTTTGTATTTTCTTTGACT NGG 10 8 17 8 24 0 100 5 10
    240605214012_pdba ACAACAGTCAAAGAAAATACAAAATGCAAAAG(+CAGTGT)GATCCTAACTCAAAACATCCAGGTAATCCAG NGG 10 8 17 8 24 0 100 5 10