Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605213918_9io5 CACACATCTGTGCACTGACTTTGCCATTGAGG(+CAGTGT)GATCCTCTGGGTTGGGTCTTTAAGACGAAGG NGG 10 8 17 8 24 0 100 5 10
    240605213916_nw0u GTTTCCCTTCGTCTTAAAGACCCAACCCAGAG(+CAGTGT)GATCCCTCAATGGCAAAGTCAGTGCACAGAT NGG 10 8 17 8 24 0 100 5 10
    240605213915_l7kk GCCCTTCCCACCTTTGCCTGTCTACAGTAGTG(+CAGTGT)GATCCATTTCCTCCCGAGGTTGCCCATCAAT NGG 10 8 17 8 24 0 100 5 10
    240605213914_1kwl ATCCAATTGATGGGCAACCTCGGGAGGAAATG(+CAGTGT)GATCCACTACTGTAGACAGGCAAAGGTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605213912_qqs2 ATACTCCCAGAATGAGCAGAGGGTCCAAAGTG(+CAGTGT)GATCCCTGAGCCATTTTGAATAATTTTATCA NGG 10 8 17 8 24 0 100 5 10
    240605213911_wfc9 AGTGGTGATAAAATTATTCAAAATGGCTCAGG(+CAGTGT)GATCCACTTTGGACCCTCTGCTCATTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605213909_2dkl TAGCACAGGGTCCAGGCAACCTCAGCCTATGG(+CAGTGT)GATCCTTAGGCACTGTGTCACAGGAACGTCC NGG 10 8 17 8 24 0 100 5 10
    240605213907_lav1 AGCTAGGACGTTCCTGTGACACAGTGCCTAAG(+CAGTGT)GATCCCATAGGCTGAGGTTGCCTGGACCCTG NGG 10 8 17 8 24 0 100 5 10
    240605213906_vnnd GTTTCAAGATTTTCTAACACAAATTTACATTG(+CAGTGT)GTACCAAAATGGGCAAGAAAATGAAGGCACA NGG 10 8 17 8 24 0 100 5 10
    240605213904_q8cc GAGCCTGTGCCTTCATTTTCTTGCCCATTTTG(+CAGTGT)GTACCAATGTAAATTTGTGTTAGAAAATCTT NGG 10 8 17 8 24 0 100 5 10