Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605213817_di2p AATTTTCCATTCCTGCATTAGTCTAGTGTATG(+CAGTGT)GATCCACGTGCTCTCTTTTAGTATTATCCTA NGG 10 8 17 8 24 0 100 5 10
    240605213815_d3zh ACTTATAGGATAATACTAAAAGAGAGCACGTG(+CAGTGT)GATCCATACACTAGACTAATGCAGGAATGGA NGG 10 8 17 8 24 0 100 5 10
    240605213814_9lbt TAAATTCAATATAAATTGTGATCTCTTGCTGG(+CAGTGT)GATCCCAGGCCCATTGTGTTTCCAGGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605213811_ki18 TCCCTACAGCCCTGGAAACACAATGGGCCTGG(+CAGTGT)GATCCCAGCAAGAGATCACAATTTATATTGA NGG 10 8 17 8 24 0 100 5 10
    240605213810_ip1v TAGGTAACAAATTTATCAATCAACTATGTTTG(+CAGTGT)GATCCAGGGAATCTCAAGTTATTCAATTCAT NGG 10 8 17 8 24 0 100 5 10
    240605213808_5p2n AGAGAATGAATTGAATAACTTGAGATTCCCTG(+CAGTGT)GATCCAAACATAGTTGATTGATAAATTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605213807_7trd TAACTACACTTCTAAGGCAGATGCTTCAGATG(+CAGTGT)GTACCCAATAAAGCCTATCAGAAAAGAGGAT NGG 10 8 17 8 24 0 100 5 10
    240605213805_pw83 AGAGCATCCTCTTTTCTGATAGGCTTTATTGG(+CAGTGT)GTACCATCTGAAGCATCTGCCTTAGAAGTGT NGG 10 8 17 8 24 0 100 5 10
    240605213804_oo23 GACGAAGCAGGTGGGCAAGGATAGAGGAAGAG(+CAGTGT)GTACCACCTGCCCGGGGGGGTGAGGGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605213802_f4t9 TTTAACAGCCCCCTCACCCCCCCGGGCAGGTG(+CAGTGT)GTACCTCTTCCTCTATCCTTGCCCACCTGCT NGG 10 8 17 8 24 0 100 5 10