Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605213848_t7m6 TGGTCCAAAAGTCAATGGCTGCAGTCCGTGTG(+CAGTGT)GATCCAATGGTAGAGACTTTTTTCTAGTACA NGG 10 8 17 8 24 0 100 5 10
    240605213846_txah AAAAGTGTACTAGAAAAAAGTCTCTACCATTG(+CAGTGT)GATCCACACGGACTGCAGCCATTGACTTTTG NGG 10 8 17 8 24 0 100 5 10
    240605213845_8055 TGCTCCTACAAGGCTCACAGAAGAAGAGCATG(+CAGTGT)GATCCCCTTCAGCAAGAATGCTTAAGACTCG NGG 10 8 17 8 24 0 100 5 10
    240605213843_y49q TGACACGAGTCTTAAGCATTCTTGCTGAAGGG(+CAGTGT)GATCCATGCTCTTCTTCTGTGAGCCTTGTAG NGG 10 8 17 8 24 0 100 5 10
    240605213842_qf6o AAAGGAAAGAGGAAAAAAAAAGGTCAAAAAAG(+CAGTGT)GTACCAAAAGTCACACACAAAAAACACAAGA NGG 10 8 17 8 24 0 100 5 10
    240605213840_8nkm AATACTCTTGTGTTTTTTGTGTGTGACTTTTG(+CAGTGT)GTACCTTTTTTGACCTTTTTTTTTCCTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605213838_nlzy AATAAAGTCAGATCATTACAATCTTAACTGTG(+CAGTGT)GTACCCTTTTTTGATCAAAAAAACCTAGTTA NGG 10 8 17 8 24 0 100 5 10
    240605213837_if8z AACAGTAACTAGGTTTTTTTGATCAAAAAAGG(+CAGTGT)GTACCACAGTTAAGATTGTAATGATCTGACT NGG 10 8 17 8 24 0 100 5 10
    240605213835_662g TTCCGACTTTATCATTTGAGGAGAGAACAAGG(+CAGTGT)GTACCGTCTTGTAATTTTGCCTTTCTTGGTA NGG 10 8 17 8 24 0 100 5 10
    240605213834_zoki AATGTTACCAAGAAAGGCAAAATTACAAGACG(+CAGTGT)GTACCCTTGTTCTCTCCTCAAATGATAAAGT NGG 10 8 17 8 24 0 100 5 10