Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605213934_fnj9 CACCAAGCCTGATATCTGTGTTTGAACCCTAG(+CAGTGT)GATCCATATGGTAGGAGAGAACTAACCCACA NGG 10 8 17 8 24 0 100 5 10
    240605213933_lyjk CAACTTGTGGGTTAGTTCTCTCCTACCATATG(+CAGTGT)GATCCTAGGGTTCAAACACAGATATCAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605213930_2o90 TGGGTTTGGACTCCTTGTCTACATCTGTCCCG(+CAGTGT)GATCCTCAACGCTCTCCAAAGGACAGCCTAC NGG 10 8 17 8 24 0 100 5 10
    240605213929_x456 CGGTGGTAGGCTGTCCTTTGGAGAGCGTTGAG(+CAGTGT)GATCCGGGACAGATGTAGACAAGGAGTCCAA NGG 10 8 17 8 24 0 100 5 10
    240605213927_mfir TATATTTAAAAAACAAAAACAGTCTTGCCATG(+CAGTGT)GATCCAGTGTTAACACTGTCATTTTGTAACA NGG 10 8 17 8 24 0 100 5 10
    240605213926_fbyj AAGGCTGTTACAAAATGACAGTGTTAACACTG(+CAGTGT)GATCCATGGCAAGACTGTTTTTGTTTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240605213925_sg1d AGACCCCACACTTTTTGTAAGACATCAATCAG(+CAGTGT)GATCCCCATATTAATTATAAGCATCACTGAA NGG 10 8 17 8 24 0 100 5 10
    240605213923_uq9v CAACATTCAGTGATGCTTATAATTAATATGGG(+CAGTGT)GATCCTGATTGATGTCTTACAAAAAGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605213921_22cu TGACACTGAAGGAATAGGCAGAGGTTTTGAAG(+CAGTGT)GTACCTCATAGCTACCACAATGATAATAACA NGG 10 8 17 8 24 0 100 5 10
    240605213920_yzn8 GTGGTTGTTATTATCATTGTGGTAGCTATGAG(+CAGTGT)GTACCTTCAAAACCTCTGCCTATTCCTTCAG NGG 10 8 17 8 24 0 100 5 10