Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605213903_yw8a CCAAAAAATACAGCAAAGACAGGAACGTCCAG(+CAGTGT)GATCCGGGTCTGTTAATAAAGCCACCTCGAG NGG 10 8 17 8 24 0 100 5 10
    240605213900_gvzq CTAGTCTCGAGGTGGCTTTATTAACAGACCCG(+CAGTGT)GATCCTGGACGTTCCTGTCTTTGCTGTATTT NGG 10 8 17 8 24 0 100 5 10
    240605213859_5986 ATCAGGCTGGGGAGGTTAGTGCACAATCAGTG(+CAGTGT)GATCCATGCCCCGCCTCCAAGCTCTGCAAAC NGG 10 8 17 8 24 0 100 5 10
    240605213858_udvn GTGGGGTTTGCAGAGCTTGGAGGCGGGGCATG(+CAGTGT)GATCCACTGATTGTGCACTAACCTCCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605213856_q065 CAGAAGTCAGAGTCATCAGTCATTTGGCTAAG(+CAGTGT)GTACCGCTGAGACACAATATCTCTGTAACTT NGG 10 8 17 8 24 0 100 5 10
    240605213855_mtk0 AAAGAAAGTTACAGAGATATTGTGTCTCAGCG(+CAGTGT)GTACCTTAGCCAAATGACTGATGACTCTGAC NGG 10 8 17 8 24 0 100 5 10
    240605213854_5492 GGAAAAAACTGAAACACTAAGTGTAGGAAGAG(+CAGTGT)GATCCTAGAAAACTAAACGTATTGACTTTGA NGG 10 8 17 8 24 0 100 5 10
    240605213852_iccw TTATTTCAAAGTCAATACGTTTAGTTTTCTAG(+CAGTGT)GATCCTCTTCCTACACTTAGTGTTTCAGTTT NGG 10 8 17 8 24 0 100 5 10
    240605213851_dgue CTTCACACAACATCTGCATTCACATATTTTAG(+CAGTGT)GATCCTTTGTCTCTCTTCCTGTACACTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605213850_2bbb AGATGAAAAAGTGTACAGGAAGAGAGACAAAG(+CAGTGT)GATCCTAAAATATGTGAATGCAGATGTTGTG NGG 10 8 17 8 24 0 100 5 10