Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605213952_bcsb TGGGTTTGTTTTCCCTGAACCCCCTCCCCCAG(+CAGTGT)GATCCAGACTTCCCACTTATATAGCTTATCA NGG 10 8 17 8 24 0 100 5 10
    240605213950_ece4 ATAGTTGATAAGCTATATAAGTGGGAAGTCTG(+CAGTGT)GATCCTGGGGGAGGGGGTTCAGGGAAAACAA NGG 10 8 17 8 24 0 100 5 10
    240605213948_4ew0 CATGTCTGTCAGGCATTTATATAAATTTTGGG(+CAGTGT)GATCCAAACTCTGGATTTTATTGTTATTTTT NGG 10 8 17 8 24 0 100 5 10
    240605213947_m1pl AGCACAAAAATAACAATAAAATCCAGAGTTTG(+CAGTGT)GATCCCCAAAATTTATATAAATGCCTGACAG NGG 10 8 17 8 24 0 100 5 10
    240605213945_3ss5 GACTTTATGTGGGAGCTGGGGATTTGAACTAG(+CAGTGT)GATCCTTGTATAGCAAGCATCCATATTCTGA NGG 10 8 17 8 24 0 100 5 10
    240605213944_44nt ATGGTTCAGAATATGGATGCTTGCTATACAAG(+CAGTGT)GATCCTAGTTCAAATCCCCAGCTCCCACATA NGG 10 8 17 8 24 0 100 5 10
    240605213943_h4vp TTGGATTAAAAGCCTGTTTATCTCCGTATTTG(+CAGTGT)GATCCCTGGATTCTATAGCCTTGCCAAAGAC NGG 10 8 17 8 24 0 100 5 10
    240605213941_pbiu GTTGAGTCTTTGGCAAGGCTATAGAATCCAGG(+CAGTGT)GATCCAAATACGGAGATAAACAGGCTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240605213938_hq99 TCAAAGACCTCAAGAACTGCCTATGGACCCAG(+CAGTGT)GTACCTTCCCAATGCATGAGCTACAAACCCA NGG 10 8 17 8 24 0 100 5 10
    240605213936_3ywh GAAGGTGGGTTTGTAGCTCATGCATTGGGAAG(+CAGTGT)GTACCTGGGTCCATAGGCAGTTCTTGAGGTC NGG 10 8 17 8 24 0 100 5 10